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Literature summary extracted from

  • Loder, A.J.; Han, Y.; Hawkins, A.B.; Lian, H.; Lipscomb, G.L.; Schut, G.J.; Keller, M.W.; Adams, M.W.; Kelly, R.M.
    Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea (2016), Metab. Eng., 38, 446-463 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.84 ATP required Metallosphaera sedula

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.84 gene Msed_1426, cloned from genomic DNA, recombinant co-expression of His-tagged enzyme with enzyme succinic semialdehyde reductase (NADPH), SSR, gene Msed_1424 from Metallosphaera sedula, in Escherichia coli strain Rosetta 2(DE3). Construction of a yeast two-hybrid assay for protein interaction analysis of ACR and SSR. ACR cannot be expressed in soluble, active form in Escherichia coli Metallosphaera sedula
4.2.1.116 gene hpcd, recombinant expression of His-tagged enzyme HPCD in Escherichi coli strain Rosetta 2 (DE3), coexpression with 3-hydroxypropionyl-CoA synthetase, acryloyl-CoA reductase, and succinic semialdehyde reductase. Yeast two-hybrid assay for protein interaction analysis of 3-hydroxypropionyl-CoA dehydratase and 3-hydroxypropionyl-CoA synthetase (HPCS-HPCD) Metallosphaera sedula

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.84 additional information metabolic engineering analysis of the the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) cycle for improving the pathway flux, overview Metallosphaera sedula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.116 additional information
-
 additional information kinetic modelling, overview Metallosphaera sedula
4.2.1.116 0.0251
-
 3-hydroxypropanoyl-CoA recombinant enzyme, pH 6.5, 65°C Metallosphaera sedula

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.84 Mg2+ required Metallosphaera sedula

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.116 220000
-
 recombinant enzyme, gel filtration Metallosphaera sedula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.84 acrylyl-CoA + NADPH + H+ Metallosphaera sedula
-
 propanoyl-CoA + NADP+
-
 r
1.3.1.84 acrylyl-CoA + NADPH + H+ Metallosphaera sedula DSM 5348
-
 propanoyl-CoA + NADP+
-
 r
4.2.1.116 3-hydroxypropanoyl-CoA Metallosphaera sedula
-
 acryloyl-CoA + H2O
-
 r
4.2.1.116 3-hydroxypropanoyl-CoA Metallosphaera sedula ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2
-
 acryloyl-CoA + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.84 Metallosphaera sedula A4YGN2
-
-
1.3.1.84 Metallosphaera sedula DSM 5348 A4YGN2
-
-
4.2.1.116 Metallosphaera sedula A4YI89
-
-
4.2.1.116 Metallosphaera sedula ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2 A4YI89
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.84 recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2(DE3) by immobilized metal affinity chromatography and gel filtration Metallosphaera sedula
4.2.1.116 recombinant His-tagged enzyme HPCD from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography Metallosphaera sedula

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.84 propanoyl-CoA + NADP+ = acryloyl-CoA + NADPH + H+ the enzyme followes an ordered Bi-Bi reaction mechanism Metallosphaera sedula
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.84 acrylyl-CoA + NADPH + H+
-
 Metallosphaera sedula propanoyl-CoA + NADP+
-
 r
1.3.1.84 acrylyl-CoA + NADPH + H+
-
 Metallosphaera sedula DSM 5348 propanoyl-CoA + NADP+
-
 r
1.3.1.84 additional information the enzyme activity is assayed coupled to 3-hydroxypropionyl-CoA synthetase (AMP-forming) Metallosphaera sedula ?
-
 ?
1.3.1.84 additional information the enzyme activity is assayed coupled to 3-hydroxypropionyl-CoA synthetase (AMP-forming) Metallosphaera sedula DSM 5348 ?
-
 ?
4.2.1.116 3-hydroxypropanoyl-CoA
-
 Metallosphaera sedula acryloyl-CoA + H2O
-
 r
4.2.1.116 3-hydroxypropanoyl-CoA
-
 Metallosphaera sedula ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2 acryloyl-CoA + H2O
-
 r
4.2.1.116 additional information coupled assay of enzyme HPCD with 3-hydroxypropionyl-CoA synthetase (HPCS) and acryloyl-CoA reductase (ACR) Metallosphaera sedula ?
-
 ?
4.2.1.116 additional information coupled assay of enzyme HPCD with 3-hydroxypropionyl-CoA synthetase (HPCS) and acryloyl-CoA reductase (ACR) Metallosphaera sedula ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.84 ? x * 36000, recombinant His-tagged enzyme, SDS-PAGE Metallosphaera sedula
1.3.1.84 More quaternary structure analysis of ACR Metallosphaera sedula
4.2.1.116 homooctamer 8 * 28000, recombinant enzyme, SDS-PAGE Metallosphaera sedula

Synonyms

EC Number Synonyms Comment Organism
1.3.1.84 Acr
-
 Metallosphaera sedula
1.3.1.84 acryloyl-CoA reductase
-
 Metallosphaera sedula
1.3.1.84 acryloyl-CoA reductase (NADPH)
-
 Metallosphaera sedula
1.3.1.84 Msed_1426
-
 Metallosphaera sedula
4.2.1.116 HPCD
-
 Metallosphaera sedula
4.2.1.116 Msed_2001
-
 Metallosphaera sedula

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.84 65
-
 assay at Metallosphaera sedula
4.2.1.116 65
-
 coupled assay at Metallosphaera sedula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.84 6.5
-
 assay at Metallosphaera sedula
4.2.1.116 6.5
-
 coupled assay at Metallosphaera sedula

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.84 NADPH
-
 Metallosphaera sedula

General Information

EC Number General Information Comment Organism
1.3.1.84 metabolism the enzyme is part of the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) cycle fixes CO2 in extremely thermoacidophilic archaea, pathway regulation and modeling, enzyme kinetics-based models, reaction kinetics model of the 3HP/4HB cycle, detailed overview Metallosphaera sedula
1.3.1.84 additional information two-hybrid assay analysis of the protein interaction of ACR and SSR in Saccharomyces cerevisiae Metallosphaera sedula
4.2.1.116 additional information yeast two-hybrid assay for protein interaction analysis of 3-hydroxypropionyl-CoA dehydratase and 3-hydroxypropionyl-CoA synthetase (HPCS-HPCD), method optimization, metabolic engineering, overview Metallosphaera sedula
4.2.1.116 physiological function 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) cycle fixes CO2 in extremely thermoacidophilic archaea and holds promise for metabolic engineering because of its thermostability and potentially rapid pathway kinetics. Modelling of the reactions and kinetics of five of the cycle enzymes: malonyl-CoA/succinyl-CoA reductase, 3-hydroxypropionyl-CoA synthetase, 3-hydroxypropionyl-CoA dehydratase, acryloyl-CoA reductase, and succinic semialdehyde reductase, that are recombinantly expressed in Escherichia coli. Reaction kinetics model of the 3HP/4HB cycle, overview Metallosphaera sedula