EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.1 | L-ascorbate | required | Homo sapiens |
EC Number | Application | Comment | Organism |
---|---|---|---|
1.14.11.1 | drug development | enzyme BBOX is a drug target for the treatment of myocardial infarction | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.1 | additional information | development and application of 1H NMR GBB/2OG reporter based assays employing paramagnetic relaxation enhancement to monitor inhibitor binding to the BBOX active site, method development and evaluation, overview. The method assesses inhibitors for competitive binding with 2-oxoglutarate or gamma-butyrobetaine, or both, and is exemplified with a set of isoquinoline-based inhibitors, structure-activity relationships. Docking simulation showing possible conformational changes as a result of binding inhibitors N-(1-chloro-4-hydroxyisoquinoline-3-carbonyl)glycine or N-(1-chloro-4-hydroxyisoquinoline-3-carbonyl)-L-tryptophan. NMR spectroscopic structures | Homo sapiens | |
1.14.11.1 | N-(1-chloro-4-hydroxyisoquinoline-3-carbonyl)-L-tryptophan | - |
Homo sapiens | |
1.14.11.1 | N-(1-chloro-4-hydroxyisoquinoline-3-carbonyl)glycine | - |
Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.1 | Fe2+ | dependent on, required for catalysis, bound to the active site | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.1 | 4-trimethylammoniobutanoate + 2-oxoglutarate + O2 | Homo sapiens | - |
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.11.1 | Homo sapiens | O75936 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.1 | 4-trimethylammoniobutanoate + 2-oxoglutarate + O2 | - |
Homo sapiens | 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.11.1 | BBOX | - |
Homo sapiens |
1.14.11.1 | gamma-butyrobetaine hydroxylase | - |
Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.11.1 | 7.5 | - |
assay at | Homo sapiens |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.14.11.1 | 0.0012 | - |
pH 7.5, temperature not specified in the publication, recombinant enzyme | Homo sapiens | N-(1-chloro-4-hydroxyisoquinoline-3-carbonyl)glycine | |
1.14.11.1 | 0.026 | - |
pH 7.5, temperature not specified in the publication, recombinant enzyme | Homo sapiens | N-(1-chloro-4-hydroxyisoquinoline-3-carbonyl)-L-tryptophan |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.11.1 | evolution | the enzyme belongs to the 2-oxoglutarate/Fe(II) dependent oxygenase family | Homo sapiens |
1.14.11.1 | physiological function | gamma-butyrobetaine hydroxylase (BBOX) is a 2-oxoglutarate-dependent oxygenase that catalyses the stereoselective C-3 hydroxylation of gamma-butyrobetaine to give L-carnitine. L-Carnitine is involved in fatty acid metabolism | Homo sapiens |