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Literature summary extracted from

  • Jung, W.; Kim, E.J.; Han, S.J.; Choi, H.G.; Kim, S.
    Characterization of stearoyl-CoA desaturases from a psychrophilic antarctic copepod, Tigriopus kingsejongensis (2016), Mar. Biotechnol., 18, 564-574 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.1 gene SCD-1, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression in Escherichia coli srain BL21(DE3) Tigriopus kingsejongensis
1.14.19.1 gene SCD-2, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression in Escherichia coli srain BL21(DE3) Tigriopus kingsejongensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.1 membrane transmembrane enzyme Tigriopus kingsejongensis 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.1 palmitoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ Tigriopus kingsejongensis
-
 palmitoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.1 stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ Tigriopus kingsejongensis
-
 oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.1 Tigriopus kingsejongensis
-
 collected in a tidal pool near the King Sejong Station, King George Island, Antarctica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.1 palmitoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Tigriopus kingsejongensis palmitoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.1 stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Tigriopus kingsejongensis oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.1 ? x * 26100, about, sequence calculation Tigriopus kingsejongensis
1.14.19.1 ? x * 43100, about, sequence calculation Tigriopus kingsejongensis

Synonyms

EC Number Synonyms Comment Organism
1.14.19.1 SCD-1
-
 Tigriopus kingsejongensis
1.14.19.1 SCD-2
-
 Tigriopus kingsejongensis
1.14.19.1 stearoyl-CoA desaturase
-
 Tigriopus kingsejongensis
1.14.19.1 TkSCD-1
-
 Tigriopus kingsejongensis
1.14.19.1 TkSCD-2
-
 Tigriopus kingsejongensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.1 cytochrome b5
-
 Tigriopus kingsejongensis

General Information

EC Number General Information Comment Organism
1.14.19.1 additional information a second transmembrane domain integrated in the membrane seems to be critical for desaturase activity Tigriopus kingsejongensis
1.14.19.1 physiological function both SCD genes from an Antarctic marine copepod encode a functional desaturase that is capable of increasing the amounts of palmitoleic acid and oleic acid in a prokaryotic expression system Tigriopus kingsejongensis