Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yuan, Y.; Hu, Y.; Zhang, H.; Leng, J.; Li, F.; Zhao, X.; Gao, J.; Zhou, Y.
    Characterization of a recombinant multifunctional glycoside hydrolase family 3 beta-xylosidase/alpha-L-arabinofuranosidase/beta-glucosidase from Cellulosimicrobium cellulans sp. 21 (2016), J. Mol. Catal. B, 131, 65-72 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 expressed in Escherichia coli BL21(DE3) cells Cellulosimicrobium cellulans
3.2.1.55 expressed in Escherichia coli BL21(DE3) cells Cellulosimicrobium cellulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 Ba2+ 31.8% residual activity at 50 mM Cellulosimicrobium cellulans
3.2.1.37 Ca2+ 20.3% residual activity at 50 mM Cellulosimicrobium cellulans
3.2.1.37 Cu2+ 0.4% residual activity at 50 mM Cellulosimicrobium cellulans
3.2.1.37 dithiothreitol 49.2% residual activity at 50 mM Cellulosimicrobium cellulans
3.2.1.37 EDTA 1.2% residual activity at 50 mM Cellulosimicrobium cellulans
3.2.1.37 Fe3+ complete inhibition at 50 mM Cellulosimicrobium cellulans
3.2.1.37 Hg2+ complete inhibition at 50 mM Cellulosimicrobium cellulans
3.2.1.37 Mn2+ 16% residual activity at 50 mM Cellulosimicrobium cellulans
3.2.1.37 SDS complete inhibition at 50 mM Cellulosimicrobium cellulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 4
-
 4-nitrophenyl beta-D-xyloside at pH 8.0 and 37°C Cellulosimicrobium cellulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.37 K+ 132.2% activity at 50 mM Cellulosimicrobium cellulans
3.2.1.37 Mg2+ 108% activity at 50 mM Cellulosimicrobium cellulans
3.2.1.37 Na+ 132.6% activity at 50 mM Cellulosimicrobium cellulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Cellulosimicrobium cellulans
-
-
-
3.2.1.37 Cellulosimicrobium cellulans 21
-
-
-
3.2.1.55 Cellulosimicrobium cellulans
-
-
-
3.2.1.55 Cellulosimicrobium cellulans 21
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 Ni Sepharose column chromatography Cellulosimicrobium cellulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 2-nitrophenyl beta-D-glucoside + H2O 6.4% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 2-nitrophenol + D-glucose
-
 ?
3.2.1.37 2-nitrophenyl beta-D-glucoside + H2O 6.4% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 21 2-nitrophenol + D-glucose
-
 ?
3.2.1.37 4-nitrophenyl alpha-D-galactoside + H2O 0.3% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 4-nitrophenol + D-galactose
-
 ?
3.2.1.37 4-nitrophenyl alpha-D-glucoside + H2O 1.5% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 4-nitrophenol + D-glucose
-
 ?
3.2.1.37 4-nitrophenyl alpha-D-glucoside + H2O 1.5% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 21 4-nitrophenol + D-glucose
-
 ?
3.2.1.37 4-nitrophenyl alpha-D-mannoside + H2O 0.4% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 4-nitrophenol + mannose
-
 ?
3.2.1.37 4-nitrophenyl alpha-L-arabinofuranoside + H2O 14.4% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-galactoside + H2O 1.4% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 4-nitrophenol + D-galactose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-galactoside + H2O 1.4% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 21 4-nitrophenol + D-galactose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-glucoside + H2O 9.6% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 4-nitrophenol + D-glucose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-glucoside + H2O 9.6% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 21 4-nitrophenol + D-glucose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xyloside + H2O 100% activity Cellulosimicrobium cellulans 4-nitrophenol + D-xylose
-
 ?
3.2.1.37 laminaribose + H2O 17.5% activity compared to xylobiose Cellulosimicrobium cellulans ?
-
 ?
3.2.1.37 laminaritriose + H2O 14.4% activity compared to xylobiose Cellulosimicrobium cellulans ?
-
 ?
3.2.1.37 additional information no activity with gentiobiose, cellobiose, and cellotriose Cellulosimicrobium cellulans ?
-
 ?
3.2.1.37 additional information no activity with gentiobiose, cellobiose, and cellotriose Cellulosimicrobium cellulans 21 ?
-
 ?
3.2.1.37 sophorose + H2O 19.9% activity compared to xylobiose Cellulosimicrobium cellulans ?
-
 ?
3.2.1.37 xylobiose + H2O 100% Cellulosimicrobium cellulans 2 D-xylose
-
 ?
3.2.1.37 xylotriose + H2O 100% activity compared to xylobiose Cellulosimicrobium cellulans xylobiose + D-xylose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O 14.4% activity compared to 4-nitrophenyl beta-D-xyloside Cellulosimicrobium cellulans 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl beta-D-xyloside + H2O 100% activity Cellulosimicrobium cellulans 4-nitrophenol + D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 ? x * 95000, SDS-PAGE Cellulosimicrobium cellulans
3.2.1.55 ? x * 95000, SDS-PAGE Cellulosimicrobium cellulans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 ccxyl3a
-
 Cellulosimicrobium cellulans
3.2.1.55 ccxyl3a gene name of multifunctional beta-xylosidase/alpha-L-arabinofuranosidase/beta-glucosidase Cellulosimicrobium cellulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 45
-
-
 Cellulosimicrobium cellulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 40
-
 the enzyme remains stable for 1 h up to 40°C Cellulosimicrobium cellulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 15.4
-
 4-nitrophenyl beta-D-xyloside at pH 8.0 and 37°C Cellulosimicrobium cellulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 8.5
-
-
 Cellulosimicrobium cellulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.37 6.5 9.5 the enzyme retains more than 60% activity between pH 6.5 and 9.5 for 24 h Cellulosimicrobium cellulans