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Literature summary extracted from

  • Li, R.; Li, N.; Wang, Z.; Wang, X.; Liu, W.
    Characterization of polyphenol oxidase from Jipicao Yam (2015), J. Food Biochem., 39, 173-178 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 Ba2+
-
Dioscorea polystachya
1.14.18.1 Ca2+
-
Dioscorea polystachya
1.14.18.1 Citric acid
-
Dioscorea polystachya
1.14.18.1 Cu2+ slight inhibition at 1 mM Dioscorea polystachya
1.14.18.1 EDTA
-
Dioscorea polystachya
1.14.18.1 K+
-
Dioscorea polystachya
1.14.18.1 L-cysteine strong inhibitor Dioscorea polystachya
1.14.18.1 Mn2+
-
Dioscorea polystachya
1.14.18.1 Na+
-
Dioscorea polystachya
1.14.18.1 NaHSO3 strong inhibitor Dioscorea polystachya
1.14.18.1 oxalic acid
-
Dioscorea polystachya
1.14.18.1 phytic acid
-
Dioscorea polystachya
1.14.18.1 Zn2+
-
Dioscorea polystachya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 additional information
-
additional information enzyme kinetics Dioscorea polystachya
1.14.18.1 1.5
-
chlorogenic acid pH 6.8, 30°C Dioscorea polystachya
1.14.18.1 4.6
-
Coke gallic acid pH 6.8, 30°C Dioscorea polystachya
1.14.18.1 6.8
-
gallic acid pH 6.8, 30°C Dioscorea polystachya
1.14.18.1 18
-
catechol pH 6.8, 30°C Dioscorea polystachya
1.14.18.1 25.6
-
guaiacolum pH 6.8, 30°C Dioscorea polystachya

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 Cu2+ a copper-containing enzyme Dioscorea polystachya
1.14.18.1 Fe3+ activates Dioscorea polystachya
1.14.18.1 Mg2+ activates Dioscorea polystachya

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.1 2 L-dopa + O2 Dioscorea polystachya
-
2 dopaquinone + 2 H2O
-
?
1.14.18.1 L-tyrosine + O2 Dioscorea polystachya
-
dopaquinone + H2O
-
?
1.14.18.1 additional information Dioscorea polystachya PPO is an enzyme concerning the o-hydroxylation of monophenols to o-diphenols acting as cresolase, EC 1.14.18.1, and the oxidation of o-diphenols to o-quinones acting as catecholase, EC 1.10.3.1 ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Dioscorea polystachya
-
Jipicao yam grown in China
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 tuber
-
Dioscorea polystachya
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 2 catechol + O2
-
Dioscorea polystachya 2 1,2-benzoquinone + 2 H2O
-
?
1.14.18.1 2 L-dopa + O2
-
Dioscorea polystachya 2 dopaquinone + 2 H2O
-
?
1.14.18.1 chlorogenic acid + O2
-
Dioscorea polystachya ?
-
?
1.14.18.1 Coke gallic acid + O2
-
Dioscorea polystachya ?
-
?
1.14.18.1 gallic acid + O2
-
Dioscorea polystachya ?
-
?
1.14.18.1 guaiacolum + O2
-
Dioscorea polystachya ?
-
?
1.14.18.1 L-tyrosine + O2
-
Dioscorea polystachya dopaquinone + H2O
-
?
1.14.18.1 additional information PPO is an enzyme concerning the o-hydroxylation of monophenols to o-diphenols acting as cresolase, EC 1.14.18.1, and the oxidation of o-diphenols to o-quinones acting as catecholase, EC 1.10.3.1 Dioscorea polystachya ?
-
?
1.14.18.1 additional information no activity with p-hydroxyphenylalanine Dioscorea polystachya ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 polyphenol oxidase
-
Dioscorea polystachya
1.14.18.1 PPO
-
Dioscorea polystachya

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 30
-
-
Dioscorea polystachya

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.18.1 20 65 activity range, profile overview Dioscorea polystachya

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.18.1 60
-
purified enzyme, stable up to Dioscorea polystachya
1.14.18.1 60 70 purified enzyme, 4 min, loss of 60% activity Dioscorea polystachya
1.14.18.1 80
-
purified enzyme, 2 min, loss of 90.5% activity Dioscorea polystachya
1.14.18.1 90 100 purified enzyme, 1 min, inactivation Dioscorea polystachya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 6.8
-
-
Dioscorea polystachya

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.18.1 4 9 activity range, profile overview Dioscorea polystachya