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Literature summary extracted from

  • Zhou, Q.; Ji, P.; Zhang, J.; Li, X.; Han, C.
    Characterization of a novel thermostable GH45 endoglucanase from Chaetomium thermophilum and its biodegradation of pectin (2017), J. Biosci. Bioeng., 124, 271-276 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Pichia pastoris Thermochaetoides thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 carboxymethylcellulose Km value 0.060 mg/ml, pH 4.0, 70°C Thermochaetoides thermophila

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Thermochaetoides thermophila V9PD44
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 glycoprotein recombinant protein expressed in Pichia pastoris Thermochaetoides thermophila

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.2.1.4 recombinant protein Thermochaetoides thermophila

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 1.52
-
 substrate carboxymethylcellulose, pH 4.0, 70°C Thermochaetoides thermophila
3.2.1.4 2.14
-
 substrate beta-1,4-D-glucan, pH 4.0, 70°C Thermochaetoides thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 beta-1,4-D-glucan + H2O
-
 Thermochaetoides thermophila ?
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Thermochaetoides thermophila ?
-
 ?
3.2.1.4 pectin + H2O
-
 Thermochaetoides thermophila galacturonic acid oligosaccharides
-
 ?
3.2.1.4 soluble starch + H2O
-
 Thermochaetoides thermophila ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 32000, SDS-PAGE, x * 24500, calculated Thermochaetoides thermophila

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 CBH45-1
-
 Thermochaetoides thermophila
3.2.1.4 CTendo45
-
 Thermochaetoides thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 70
-
-
 Thermochaetoides thermophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 60
-
 1 h, stabel Thermochaetoides thermophila
3.2.1.4 70
-
 1 h, 79% residual activtiy Thermochaetoides thermophila
3.2.1.4 80
-
 1 h, 66% residual activity Thermochaetoides thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4
-
-
 Thermochaetoides thermophila

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.4 Thermochaetoides thermophila calculated
-
 5.9