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Literature summary extracted from

  • Forsberg, Z.; Bissaro, B.; Gullesen, J.; Dalhus, B.; Vaaje-Kolstad, G.; Eijsink, V.G.H.
    Structural determinants of bacterial lytic polysaccharide monooxygenase functionality (2018), J. Biol. Chem., 293, 1397-1412 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.53
-
 Streptomyces coelicolor
1.14.99.53
-
 Micromonospora aurantiaca
1.14.99.54
-
 Streptomyces coelicolor
1.14.99.54
-
 Micromonospora aurantiaca
1.14.99.56
-
 Streptomyces coelicolor
1.14.99.56
-
 Micromonospora aurantiaca

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.99.53 structure of the catalytic domain, residues 37-230, to 1.08 A resolution. The active site in is formed by residues His-37 and His-144 that coordinate the copper atom in a T-shaped geometry Streptomyces coelicolor
1.14.99.53 structure of the catalytic domain, residues 37-230, to 1.08 A resolution. The active site in is formed by residues His-37 and His-144 that coordinate the copper atom in a T-shaped geometry Micromonospora aurantiaca
1.14.99.54 structure of the catalytic domain, residues 37-230, to 1.08 A resolution. The active site in is formed by residues His-37 and His-144 that coordinate the copper atom in a T-shaped geometry Streptomyces coelicolor
1.14.99.54 structure of the catalytic domain, residues 37-230, to 1.08 A resolution. The active site in is formed by residues His-37 and His-144 that coordinate the copper atom in a T-shaped geometry Micromonospora aurantiaca
1.14.99.56 structure of the catalytic domain, residues 37-230, to 1.08 A resolution. The active site in is formed by residues His-37 and His-144 that coordinate the copper atom in a T-shaped geometry Streptomyces coelicolor
1.14.99.56 structure of the catalytic domain, residues 37-230, to 1.08 A resolution. The active site in is formed by residues His-37 and His-144 that coordinate the copper atom in a T-shaped geometry Micromonospora aurantiaca

Protein Variants

EC Number Protein Variants Comment Organism
1.14.99.53 A148G mutation leads to loss of C4 oxidation, i.e to the activity of EC 1.14.99.54 Streptomyces coelicolor
1.14.99.53 A148S mutation leads to loss of C4 oxidation, i.e to the activity of EC 1.14.99.54 Streptomyces coelicolor
1.14.99.53 D140A mutant shows moderately reduced activity and essentially unchanged oxidative regioselectivity Micromonospora aurantiaca
1.14.99.53 additional information neither truncation of theLPMO10B family 2 carbohydrate-binding module nor mutations altering access to the solvent-exposed axial copper coordination site significantly change the C1:C4 oxidation ratio Micromonospora aurantiaca
1.14.99.53 N85F mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 5.9 Micromonospora aurantiaca
1.14.99.53 W82Y mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 2.0 Micromonospora aurantiaca
1.14.99.53 W82Y/N85F mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 10.9 Micromonospora aurantiaca
1.14.99.53 W82Y/N85F/Q141W mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 5.1 Micromonospora aurantiaca
1.14.99.53 W82Y/N85F/Y116F mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 14.7 Micromonospora aurantiaca
1.14.99.53 W82Y/N85F/Y116F/Q141W mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 5.8 Micromonospora aurantiaca
1.14.99.53 W88Y/N91F mutation leads to loss of C4 oxidation, i.e to the activity of EC 1.14.99.54 Streptomyces coelicolor
1.14.99.53 Y116F no changes in the C1:C4 oxidation ratio Micromonospora aurantiaca
1.14.99.54 A148G mutation leads to loss of C4 oxidation, i.e to the activity of EC 1.14.99.54 Streptomyces coelicolor
1.14.99.54 A148S mutation leads to loss of C4 oxidation, i.e to the activity of EC 1.14.99.54 Streptomyces coelicolor
1.14.99.54 D140A mutant shows moderately reduced activity and essentially unchanged oxidative regioselectivity Micromonospora aurantiaca
1.14.99.54 additional information neither truncation of the LPMO10B family 2 carbohydrate-binding module nor mutations altering access to the solvent-exposed axial copper coordination site significantly change the C1:C4 oxidation ratio Micromonospora aurantiaca
1.14.99.54 N85F mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 5.9 Micromonospora aurantiaca
1.14.99.54 W82Y mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 2.0 Micromonospora aurantiaca
1.14.99.54 W82Y/N85F mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 10.9 Micromonospora aurantiaca
1.14.99.54 W82Y/N85F/Q141W mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 5.1 Micromonospora aurantiaca
1.14.99.54 W82Y/N85F/Y116F mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 14.7 Micromonospora aurantiaca
1.14.99.54 W82Y/N85F/Y116F/Q141W mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 5.8 Micromonospora aurantiaca
1.14.99.54 W88Y/N91F mutation leads to loss of C4 oxidation, i.e to the activity of EC 1.14.99.54 Streptomyces coelicolor
1.14.99.54 Y116F no changes in the C1:C4 oxidation ratio Micromonospora aurantiaca
1.14.99.56 A148G mutation leads to loss of C4 oxidation, i.e to the activity of EC 1.14.99.54 Streptomyces coelicolor
1.14.99.56 A148S mutation leads to loss of C4 oxidation, i.e to the activity of EC 1.14.99.54 Streptomyces coelicolor
1.14.99.56 D140A mutant shows moderately reduced activity and essentially unchanged oxidative regioselectivity Micromonospora aurantiaca
1.14.99.56 additional information neither truncation of the LPMO10B family 2 carbohydrate-binding module nor mutations altering access to the solvent-exposed axial copper coordination site significantly change the C1:C4 oxidation ratio Micromonospora aurantiaca
1.14.99.56 N85F mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 5.9 Micromonospora aurantiaca
1.14.99.56 W82Y mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 2.0 Micromonospora aurantiaca
1.14.99.56 W82Y/N85F mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 10.9 Micromonospora aurantiaca
1.14.99.56 W82Y/N85F/Q141W mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 5.1 Micromonospora aurantiaca
1.14.99.56 W82Y/N85F/Y116F mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 14.7 Micromonospora aurantiaca
1.14.99.56 W82Y/N85F/Y116F/Q141W mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 5.8 Micromonospora aurantiaca
1.14.99.56 W88Y/N91F mutation leads to loss of C4 oxidation, i.e to the activity of EC 1.14.99.54 Streptomyces coelicolor
1.14.99.56 Y116F no changes in the C1:C4 oxidation ratio Micromonospora aurantiaca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.53 Cu2+
-
 Streptomyces coelicolor
1.14.99.53 Cu2+ the active site in is formed by residues His-37 and His-144 that coordinate the copper atom in a T-shaped geometry Micromonospora aurantiaca
1.14.99.54 Cu2+
-
 Streptomyces coelicolor
1.14.99.54 Cu2+ the active site in is formed by residues His-37 and His-144 that coordinate the copper atom in a T-shaped geometry Micromonospora aurantiaca
1.14.99.56 Cu2+
-
 Streptomyces coelicolor
1.14.99.56 Cu2+ the active site in is formed by residues His-37 and His-144 that coordinate the copper atom in a T-shaped geometry Micromonospora aurantiaca

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.53 Micromonospora aurantiaca D9SZQ3
-
-
1.14.99.53 Micromonospora aurantiaca DSM 43813 D9SZQ3
-
-
1.14.99.53 Streptomyces coelicolor Q9RJC1
-
-
1.14.99.53 Streptomyces coelicolor ATCC BAA-471 Q9RJC1
-
-
1.14.99.54 Micromonospora aurantiaca D9SZQ3
-
-
1.14.99.54 Micromonospora aurantiaca DSM 43813 D9SZQ3
-
-
1.14.99.54 Streptomyces coelicolor Q9RJC1
-
-
1.14.99.54 Streptomyces coelicolor ATCC BAA-471 Q9RJC1
-
-
1.14.99.56 Micromonospora aurantiaca D9SZQ3
-
-
1.14.99.56 Micromonospora aurantiaca DSM 43813 D9SZQ3
-
-
1.14.99.56 Streptomyces coelicolor Q9RJC1
-
-
1.14.99.56 Streptomyces coelicolor ATCC BAA-471 Q9RJC1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.53 chitin + ascorbic acid + O2
-
 Streptomyces coelicolor ? + dehydroascorbate + H2O
-
 ?
1.14.99.53 chitin + ascorbic acid + O2
-
 Micromonospora aurantiaca ? + dehydroascorbate + H2O
-
 ?
1.14.99.53 chitin + ascorbic acid + O2
-
 Streptomyces coelicolor ATCC BAA-471 ? + dehydroascorbate + H2O
-
 ?
1.14.99.53 chitin + ascorbic acid + O2
-
 Micromonospora aurantiaca DSM 43813 ? + dehydroascorbate + H2O
-
 ?
1.14.99.53 phosphoric acid swollen cellulase + ascorbic acid + O2
-
 Streptomyces coelicolor ? + dehydroascorbate + H2O
-
 ?
1.14.99.53 phosphoric acid swollen cellulase + ascorbic acid + O2
-
 Micromonospora aurantiaca ? + dehydroascorbate + H2O
-
 ?
1.14.99.53 phosphoric acid swollen cellulase + ascorbic acid + O2
-
 Streptomyces coelicolor ATCC BAA-471 ? + dehydroascorbate + H2O
-
 ?
1.14.99.53 phosphoric acid swollen cellulase + ascorbic acid + O2
-
 Micromonospora aurantiaca DSM 43813 ? + dehydroascorbate + H2O
-
 ?
1.14.99.54 chitin + ascorbic acid + O2
-
 Streptomyces coelicolor ? + dehydroascorbate + H2O
-
 ?
1.14.99.54 chitin + ascorbic acid + O2
-
 Micromonospora aurantiaca ? + dehydroascorbate + H2O
-
 ?
1.14.99.54 chitin + ascorbic acid + O2
-
 Streptomyces coelicolor ATCC BAA-471 ? + dehydroascorbate + H2O
-
 ?
1.14.99.54 chitin + ascorbic acid + O2
-
 Micromonospora aurantiaca DSM 43813 ? + dehydroascorbate + H2O
-
 ?
1.14.99.54 phosphoric acid swollen cellulase + ascorbic acid + O2
-
 Streptomyces coelicolor ? + dehydroascorbate + H2O
-
 ?
1.14.99.54 phosphoric acid swollen cellulase + ascorbic acid + O2
-
 Micromonospora aurantiaca ? + dehydroascorbate + H2O
-
 ?
1.14.99.54 phosphoric acid swollen cellulase + ascorbic acid + O2
-
 Streptomyces coelicolor ATCC BAA-471 ? + dehydroascorbate + H2O
-
 ?
1.14.99.54 phosphoric acid swollen cellulase + ascorbic acid + O2
-
 Micromonospora aurantiaca DSM 43813 ? + dehydroascorbate + H2O
-
 ?
1.14.99.56 chitin + ascorbic acid + O2
-
 Streptomyces coelicolor ? + dehydroascorbate + H2O
-
 ?
1.14.99.56 chitin + ascorbic acid + O2
-
 Micromonospora aurantiaca ? + dehydroascorbate + H2O
-
 ?
1.14.99.56 chitin + ascorbic acid + O2
-
 Streptomyces coelicolor ATCC BAA-471 ? + dehydroascorbate + H2O
-
 ?
1.14.99.56 chitin + ascorbic acid + O2
-
 Micromonospora aurantiaca DSM 43813 ? + dehydroascorbate + H2O
-
 ?
1.14.99.56 phosphoric acid swollen cellulase + ascorbic acid + O2
-
 Streptomyces coelicolor ? + dehydroascorbate + H2O
-
 ?
1.14.99.56 phosphoric acid swollen cellulase + ascorbic acid + O2
-
 Micromonospora aurantiaca ? + dehydroascorbate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.53 chitin-binding domain 3 protein
-
 Micromonospora aurantiaca
1.14.99.53 LPMO10B
-
 Streptomyces coelicolor
1.14.99.53 LPMO10B
-
 Micromonospora aurantiaca
1.14.99.53 Micau_1630
-
 Micromonospora aurantiaca
1.14.99.53 SCO0643
-
 Streptomyces coelicolor
1.14.99.54 chitin-binding domain 3 protein
-
 Micromonospora aurantiaca
1.14.99.54 LPMO10B
-
 Streptomyces coelicolor
1.14.99.54 LPMO10B
-
 Micromonospora aurantiaca
1.14.99.54 Micau_1630
-
 Micromonospora aurantiaca
1.14.99.54 SCO0643
-
 Streptomyces coelicolor
1.14.99.56 chitin-binding domain 3 protein
-
 Micromonospora aurantiaca
1.14.99.56 LPMO10B
-
 Streptomyces coelicolor
1.14.99.56 LPMO10B
-
 Micromonospora aurantiaca
1.14.99.56 Micau_1630
-
 Micromonospora aurantiaca
1.14.99.56 SCO0643
-
 Streptomyces coelicolor