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Literature summary extracted from

  • Kim, D.H.; Hino, R.; Adachi, Y.; Kobori, A.; Taketani, S.
    The enzyme engineering of mutant homodimer and heterodimer of coproporphyinogen oxidase contributes to new insight into hereditary coproporphyria and harderoporphyria (2013), J. Biochem., 154, 551-559 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.3 recombinant coexpression of HA-tagged wild-type enzyme from healthy donors and of His-tagged mutant enzyme from HCP patients in Escherichia coli strain BL21, the His-enzyme forms a heterodimer in association with the HA-tagged enzyme, The monomeric form of mutated CPOX does not show any activity and homodimeric enzymes derived from HCP mutant show low activity (about 20% of the control). The chimeric heterodimers with wild-type and mutated subunits from HCP patients show low protoporphyrinogen producing activity. The active site of the enzyme involved in the second step of decarboxylation is encoded in exon 6 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.3.3.3 D400A site-directed mutagenesis, the inactive mutant D400A remains in a monomeric form Homo sapiens
1.3.3.3 G402D site-directed mutagenesis, the mutant enzyme forms dimers Homo sapiens
1.3.3.3 K404E a naturally occuring mutant derived from patients with harderoporphyria, the mutant produces less harderoporphyrinogen. The K404E mutation leads to diminishment of the second step of the decarboxylation reaction during the conversion of coproporphyrinogen to protoporphyrinogen. The mutant enzyme forms dimers Homo sapiens
1.3.3.3 K404H site-directed mutagenesis, the mutant produces a high level of harderoporphyrinogen with low production of protoporphyrinogen similar to mutant K404E Homo sapiens
1.3.3.3 K404Q site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme Homo sapiens
1.3.3.3 additional information enzyme engineering of mutant homodimer and heterodimer of coproporphyinogen oxidase. The His-tagged mutant enzyme forms a heterodimer in association with the HA-tagged wild-type enzyme, The monomeric form of mutated CPOX does not show any activity and homodimeric enzymes derived from hereditary coproporphyria (HCP) mutant show low activity (about 20% of the control). The chimeric heterodimers with wild-type and mutated subunits from HCP patients show low protoporphyrinogen producing activity. Some mutations of amino acids 401-404 are associated with marked accumulation of reaction intermediate harderoporphyrinogen, with a decrease in the production of protoporphyrinogen, whereas K404E derived from patients with harderoporphyria produces less harderoporphyrinogen. Functional analysis of heterodimer of mutant/wild-type complex, heterophilic forms of His-R388W/HA-wild-type, His-R391W/HA-wild-type, His-D400A/HA-wild-type, His-R401W/HA-wild-type, His-G402D/HA-wild-type and His-K404E/HA-wild-type, overview Homo sapiens
1.3.3.3 R388W site-directed mutagenesis, the mutant enzyme forms dimers Homo sapiens
1.3.3.3 R391W site-directed mutagenesis, the mutant enzyme forms dimers Homo sapiens
1.3.3.3 R401W site-directed mutagenesis, the mutant enzyme forms dimers Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.3 74000
-
 recombinant wild-type enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.3 coproporphyrinogen III + O2 + 2 H+ Homo sapiens via harderoporphyrinogen intermediate production, overall reaction protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.3 Homo sapiens P36551
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.3 recombinant HA-tagged wild-type enzyme and His-tagged mutant enzyme from Escherichia coli by nickel and heparin affinity chromatography, respectively Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.3 coproporphyrinogen III + O2 + 2 H+ via harderoporphyrinogen intermediate production, overall reaction Homo sapiens protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.3 homodimer 2 * 37000, about, recombinant wild-type enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.3.3.3 coproporphyinogen oxidase
-
 Homo sapiens
1.3.3.3 CPOX
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.3.3 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.3 7.4
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
1.3.3.3 malfunction partial deficiency of the enzyme coproporphyrinogen oxidase (CPOX) causes hereditary coproporphyria (HCP), an autosomal dominant-inherited disease of heme biosynthesis. Patients suffering HCP show 50% of normal activity and those with the rare autosomal recessive harderoporphyria accumulate harderoporphyrinogen, an intermediate porphyrin of the CPOX reaction. As only patients with mutation K404E in this region develop harderoporphyria, the K404E mutation leads to diminishment of the second step of the decarboxylation reaction during the conversion of coproporphyrinogen to protoporphyrinogen, implying that the active site of the enzyme involved in the second step of decarboxylation is encoded in exon 6 Homo sapiens
1.3.3.3 additional information the active site of the enzyme involved in the second step of decarboxylation is encoded in exon 6. Aspartic acid at position 400 can be a prerequisite for dimerization of enzyme CPOX Homo sapiens