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Literature summary extracted from
- Cloning, expression and biochemical characterization of recombinant superoxide dismutase from Antarctic psychrophilic bacterium Pseudoalteromonas sp. ANT506 (2016), J. Basic Microbiol., 56, 753-761 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.15.1.1 | DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Pseudoalteromonas sp. ANT506 |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.15.1.1 | chloroform | recombinant PsSOD is sensitive to a mixture of chloroform and ethanol in the ratio 3:5. Mixing the recombinant PsSOD enzyme and the solvent mixture by 2:1 and 1:1, the relative activity is 36.4 and 13.6%, respectively. In addition, no enzymatic activity remains after incubation at 1:2 mixture | Pseudoalteromonas sp. ANT506 |  |
| 1.15.1.1 | ethanol | recombinant PsSOD is sensitive to a mixture of chloroform and ethanol in the ratio 3:5. Mixing the recombinant PsSOD enzyme and the solvent mixture by 2:1 and 1:1, the relative activity is 36.4 and 13.6%, respectively. In addition, no enzymatic activity remains after incubation at 1:2 mixture | Pseudoalteromonas sp. ANT506 | |
| 1.15.1.1 | Fe3+ | 9.7% inhibition | Pseudoalteromonas sp. ANT506 | |
| 1.15.1.1 | H2O2 | preincubation in 5 mM H2O2 for 30 min results in a loss of 91% activity | Pseudoalteromonas sp. ANT506 | |
| 1.15.1.1 | NaCl | 60% activity remains at 2.5 M NaCl | Pseudoalteromonas sp. ANT506 | |
| 1.15.1.1 | Ni2+ | 33.7% inhibition | Pseudoalteromonas sp. ANT506 | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.15.1.1 | Ca2+ | activates | Pseudoalteromonas sp. ANT506 | |
| 1.15.1.1 | Cu2+ | activates | Pseudoalteromonas sp. ANT506 | |
| 1.15.1.1 | Fe2+ | a Fe/Mn-SOD | Pseudoalteromonas sp. ANT506 | |
| 1.15.1.1 | Mg2+ | activates | Pseudoalteromonas sp. ANT506 | |
| 1.15.1.1 | Mn2+ | a Fe/Mn-SOD | Pseudoalteromonas sp. ANT506 | |
| 1.15.1.1 | Zn2+ | activates | Pseudoalteromonas sp. ANT506 | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.15.1.1 | 2 superoxide + 2 H+ | Pseudoalteromonas sp. ANT506 | - |
O2 + H2O2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.15.1.1 | Pseudoalteromonas sp. ANT506 | A0A0S2E8T0 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.15.1.1 | recombinant His-tagged enzyme 12.6fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudoalteromonas sp. ANT506 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.15.1.1 | 2 superoxide + 2 H+ | - |
Pseudoalteromonas sp. ANT506 | O2 + H2O2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.15.1.1 | monomer | 1 * 21400, about, sequence calculation, 1 * 27000, recombinant His-tagged enzyme, SDS-PAGE | Pseudoalteromonas sp. ANT506 |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.15.1.1 | Fe/Mn-SOD | - |
Pseudoalteromonas sp. ANT506 |
| 1.15.1.1 | PsSOD | - |
Pseudoalteromonas sp. ANT506 |
| 1.15.1.1 | SOD | - |
Pseudoalteromonas sp. ANT506 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 30 | - |
recombinant His-tagged enzyme | Pseudoalteromonas sp. ANT506 |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | - |
50 | activity range, 13.9% of maximal activity at 0°C, 25.7% at 10°C | Pseudoalteromonas sp. ANT506 |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 40 | - |
purified recombinant His-tagged enzyme, 40 min, 82.2% activity remains | Pseudoalteromonas sp. ANT506 |
| 1.15.1.1 | 50 | - |
purified recombinant His-tagged enzyme, 40 min, 59.4% activity remains | Pseudoalteromonas sp. ANT506 |
| 1.15.1.1 | 70 | - |
purified recombinant His-tagged enzyme, 20 min, almost no activity remains | Pseudoalteromonas sp. ANT506 |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 8 | - |
recombinant His-tagged enzyme | Pseudoalteromonas sp. ANT506 |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.15.1.1 | 5 | 10 | over 90% of maximal enzyme activity at pH 7.0-9.0, rapid loss of activity below pH 5.0 and above pH 10.0 | Pseudoalteromonas sp. ANT506 |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.15.1.1 | Pseudoalteromonas sp. ANT506 | sequence calculation | - |
5.09 |
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