EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.98 | gene murB or alr5066, construction of plasmid pPJAV308 and recombinant expression of the enzyme in Escherichia coli, functional complementation of a MurB enzyme-deficient Escherchia coli mutant | Anabaena sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.1.98 | additional information | construction of a murB null mutant strain UHM350, introduction of PpetE-murB (pPJAV329) into the murB mutant in the presence of copper is sufficient for complete segregation of the murB insertion and created a viable strain UHM350, which displays a growth defect and spontaneously lyses if not passaged every 2 weeks once a sufficient density is reached during plate-based culture containing copper. The pattern of functional heterocysts and average filament lengths is altered and impaired compared to the wild-type | Anabaena sp. |
6.3.2.8 | additional information | screening and identification of a transposon mutant impaired in diazotrophic growth, the mutant strain contains a transposon insertion at nucleotide position -476 relative to the annotated translational start site of gene alr5065. The mutant, designated C6, is unable to grow diazotrophically and displays a deficit in heterocyst differentiation, producing 4.5% heterocysts compared to the 9% produced by the wild type. Proper heterocyst production is restored in mutant C6 when the mutation is complemented with both alr5065 and alr5066, encoding enzymes MurC and MurB, as PpetE-murC/B (pPJAV328) in the presence of copper, but not when either gene is introduced individually, which suggests that alr5065 and alr5066 may be in an operon and the transposon insertion affectes the expression of both genes. After repeated rounds of subculturing, C6 loses viability and ceases to grow in culture. Generation of murC mutant strain UHM351, in which murC is inactivated by pPJAV309, phenotype, overview | Nostoc sp. PCC 7120 = FACHB-418 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.8 | Mg2+ | required | Nostoc sp. PCC 7120 = FACHB-418 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.8 | ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine | Nostoc sp. PCC 7120 = FACHB-418 | - |
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine | - |
? | |
6.3.2.8 | ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine | Nostoc sp. PCC 7120 = FACHB-418 SAG 25.82 / UTEX 2576 | - |
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.98 | Anabaena sp. | Q8YM74 | i.e. Nostoc sp. | - |
1.3.1.98 | Anabaena sp. PCC 7120 | Q8YM74 | i.e. Nostoc sp. | - |
6.3.2.8 | Nostoc sp. PCC 7120 = FACHB-418 | Q8YM75 | - |
- |
6.3.2.8 | Nostoc sp. PCC 7120 = FACHB-418 SAG 25.82 / UTEX 2576 | Q8YM75 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.8 | ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine | - |
Nostoc sp. PCC 7120 = FACHB-418 | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine | - |
? | |
6.3.2.8 | ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine | - |
Nostoc sp. PCC 7120 = FACHB-418 SAG 25.82 / UTEX 2576 | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.98 | alr5066 | - |
Anabaena sp. |
1.3.1.98 | MurB | - |
Anabaena sp. |
1.3.1.98 | UDP-N-acetylenolpyruvoylglucosamine reductase | - |
Anabaena sp. |
6.3.2.8 | alr5065 | - |
Nostoc sp. PCC 7120 = FACHB-418 |
6.3.2.8 | MurC | - |
Nostoc sp. PCC 7120 = FACHB-418 |
6.3.2.8 | UDP-N-acetylmuramate:L-alanine ligase | - |
Nostoc sp. PCC 7120 = FACHB-418 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.8 | ATP | - |
Nostoc sp. PCC 7120 = FACHB-418 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.3.1.98 | malfunction | cells depleted of either murC or murB expression fail to differentiate heterocysts under normally inducing conditions and display decreased filament integrity | Anabaena sp. |
1.3.1.98 | physiological function | the multicellular bacterium Anabaena, which differentiates a periodic pattern of specialized heterocyst cells, requires peptidoglycan synthesis by the murine ligase genes murC (alr5065) and murB (alr5066) for maintenance of patterned gene expression, filament integrity, and overall development. Recombinant expression of gene murB in an MurB enzyme-deficient Escherchia coli mutant can functionally complement the mutant strain. Gene murB depletion does not affect the pattern of patS expression | Anabaena sp. |
6.3.2.8 | malfunction | mutation of the gene murC impairs heterocyst differentiation in Nostoc sp. PCC 7120. Gene murC depletion leads to aberrant expression of patS in all cells of the filament | Nostoc sp. PCC 7120 = FACHB-418 |
6.3.2.8 | metabolism | enzyme MurC catalyzes the third step in peptidoglycan biosynthesis | Nostoc sp. PCC 7120 = FACHB-418 |
6.3.2.8 | physiological function | the enzyme is important in peptidoglycan biosynthesis. Heterocyst development has been shown to require proper peptidoglycan remodeling. Determination of a direct link between peptidoglycan synthesis and the maintenance of a biological pattern in a multicellular organism. Nostoc sp. PCC 7120 differentiates a periodic pattern of specialized heterocyst cells, requiring peptidoglycan synthesis by the murine ligase genes murC (alr5065) and murB (alr5066) for maintenance of patterned gene expression, filament integrity, and overall development, overview | Nostoc sp. PCC 7120 = FACHB-418 |