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Literature summary extracted from

  • Drummond, C.; Molina, M.; Taliansky, S.; Breidenbach, C.; Fioravanti, C.
    Effects of quinizarin and five synthesized derivatives on fifth larval instar midgut ecdysone 20-monooxygenase activity of the tobacco hornworm Manduca sexta (2014), Int. J. Zool., 2014, 261512 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.99.22 9-methoxy-1,4-anthraquinone about 125% activity at 0.001 mM Manduca sexta

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.99.22 1,4-anthraquinone about 20% residual activity at 10 mM Manduca sexta
1.14.99.22 2-methoxy-1,4-anthraquinone about 18% residual activity at 1 mM Manduca sexta
1.14.99.22 9-hydroxy-1,4-anthraquinone about 40% residual activity at 10 mM Manduca sexta
1.14.99.22 9-methoxy-1,4-anthraquinone about 45% residual activity at 10 mM Manduca sexta
1.14.99.22 additional information 2-hydroxy-1,4-anthraquinone is essentially without effect on enzyme activity Manduca sexta
1.14.99.22 quinizarin about 60% residual activity at 10 mM Manduca sexta

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.22 Manduca sexta
-
 tobacco hornworm
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.99.22 larva
-
 Manduca sexta
-
1.14.99.22 midgut fifth larval instar midgut Manduca sexta
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.22 ecdysone + NADPH + O2
-
 Manduca sexta 20-hydroxyecdysone + NADP+ + H+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.22 E20M
-
 Manduca sexta
1.14.99.22 ecdysone 20-monooxygenase
-
 Manduca sexta

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.99.22 0.04
-
 at pH 7.5 and 35°C Manduca sexta 2-methoxy-1,4-anthraquinone
1.14.99.22 0.3
-
 at pH 7.5 and 35°C Manduca sexta 1,4-anthraquinone
1.14.99.22 1
-
 at pH 7.5 and 35°C Manduca sexta 9-hydroxy-1,4-anthraquinone
1.14.99.22 2
-
 at pH 7.5 and 35°C Manduca sexta 9-methoxy-1,4-anthraquinone
1.14.99.22 60
-
 at pH 7.5 and 35°C Manduca sexta quinizarin