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Literature summary extracted from

  • Liang, J.; Han, Q.; Ding, H.; Li, J.
    Biochemical identification of residues that discriminate between 3,4-dihydroxyphenylalanine decarboxylase and 3,4-dihydroxyphenylacetaldehyde synthase-mediated reactions (2017), Insect Biochem. Mol. Biol., 91, 34-43 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.107 expressed in Escherichia coli ER2566 cells Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.107 C194S the mutant shows about wild type activity Drosophila melanogaster
4.1.1.107 N192H the mutant shows increased L-dopa decarboxylase activity compared to the wild type enzyme Drosophila melanogaster
4.1.1.107 Y80F the mutant shows about wild type activity Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.107 0.88
-
 L-Dopa wild type enzyme, pH and temperature not specified in the publication Drosophila melanogaster
4.1.1.107 1.23
-
 L-Dopa mutant enzyme N192H, pH and temperature not specified in the publication Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.107 L-Dopa + O2 + H2O Drosophila melanogaster
-
 3,4-dihydroxyphenylacetaldehyde + CO2 + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.107 Drosophila melanogaster Q9VIZ9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.107 anion exchange column chromatography and gel filtration Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.107 L-Dopa + O2 + H2O
-
 Drosophila melanogaster 3,4-dihydroxyphenylacetaldehyde + CO2 + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.107 DHPA synthase
-
 Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.107 pyridoxal 5'-phosphate
-
 Drosophila melanogaster