EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.1.1.21 | D175E | the mutant with reduced aminoacylation activity is associated with Charcot-Marie-Tooth disease type 2W | Homo sapiens |
6.1.1.21 | D364Y | the mutant with reduced aminoacylation activity is associated with Charcot-Marie-Tooth disease type 2W | Homo sapiens |
6.1.1.21 | P134H | the mutant with reduced aminoacylation activity is associated with Charcot-Marie-Tooth disease type 2W | Homo sapiens |
6.1.1.21 | R137Q | the mutant with reduced aminoacylation activity is associated with Charcot-Marie-Tooth disease type 2W | Homo sapiens |
6.1.1.21 | S356N | the mutant with reduced aminoacylation activity is associated with Charcot-Marie-Tooth disease type 2W | Homo sapiens |
6.1.1.21 | T132I | the mutant with reduced aminoacylation activity is associated with Charcot-Marie-Tooth disease type 2W | Homo sapiens |
6.1.1.21 | V155G | the mutant with reduced aminoacylation activity is associated with Charcot-Marie-Tooth disease type 2W | Homo sapiens |
6.1.1.21 | Y330C | the mutant with reduced aminoacylation activity is associated with Charcot-Marie-Tooth disease type 2W | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.21 | 0.000199 | - |
tRNAHis | mutant enzyme S365N, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 0.00033 | - |
tRNAHis | mutant enzyme Y330C, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 0.000782 | - |
tRNAHis | wild type enzyme, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 0.000979 | - |
tRNAHis | mutant enzyme V155G, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 0.008 | - |
L-histidine | wild type enzyme, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 0.0108 | - |
L-histidine | mutant enzyme S365N, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 0.0442 | - |
ATP | wild type enzyme, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 0.2029 | - |
L-histidine | mutant enzyme Y330C, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 0.6872 | - |
L-histidine | mutant enzyme V155G, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 1.763 | - |
ATP | mutant enzyme Y330C, at pH 7.5 and 25°C | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.21 | ATP + L-histidine + tRNAHis | Homo sapiens | - |
AMP + diphosphate + L-histidyl-tRNAHis | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.21 | Homo sapiens | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.21 | ATP + L-histidine + tRNAHis | - |
Homo sapiens | AMP + diphosphate + L-histidyl-tRNAHis | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.1.1.21 | homodimer | 2 * 118500, sedimentation velocity analysis | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.21 | HARS | - |
Homo sapiens |
6.1.1.21 | Histidyl-tRNA synthetase | - |
Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.21 | 51.7 | - |
melting temperature | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.21 | 0.3 | - |
tRNAHis | mutant enzyme S365N, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 0.39 | - |
L-histidine | mutant enzyme S365N, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 0.48 | - |
ATP | mutant enzyme Y330C, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 0.56 | - |
tRNAHis | mutant enzyme Y330C, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 0.74 | - |
L-histidine | mutant enzyme Y330C, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 2.08 | - |
L-histidine | mutant enzyme V155G, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 3.05 | - |
tRNAHis | mutant enzyme V155G, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 4.1 | - |
L-histidine | wild type enzyme, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 5.4 | - |
tRNAHis | wild type enzyme, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 5.8 | - |
ATP | wild type enzyme, at pH 7.5 and 25°C | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.21 | ATP | - |
Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
6.1.1.21 | malfunction | mutations in histidyl-tRNA synthetase cause the dominant axonal peripheral neuropathy Charcot-Marie-Tooth disease type 2W | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.21 | 0.15 | - |
ATP | mutant enzyme S365N, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 0.272 | - |
ATP | mutant enzyme Y330C, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 0.38 | - |
ATP | mutant enzyme V155G, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 3 | - |
L-histidine | mutant enzyme V155G, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 4 | - |
L-histidine | mutant enzyme Y330C, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 40 | - |
L-histidine | mutant enzyme S365N, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 130 | - |
ATP | wild type enzyme, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 500 | - |
L-histidine | wild type enzyme, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 1500 | - |
tRNAHis | mutant enzyme S365N, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 1700 | - |
tRNAHis | mutant enzyme Y330C, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 3100 | - |
tRNAHis | mutant enzyme V155G, at pH 7.5 and 25°C | Homo sapiens | |
6.1.1.21 | 6900 | - |
tRNAHis | wild type enzyme, at pH 7.5 and 25°C | Homo sapiens |