Literature summary extracted from
Muhr, E.; Leicht, O.; Sierra, S.; Thanbichler, M.; Heider, J.
A fluorescent bioreporter for acetophenone and 1-phenylethanol derived from a specifically induced catabolic operon (2016), Front. Microbiol., 6, 1561 .
Application
EC Number |
Application |
Comment |
Organism |
---|
6.4.1.8 |
analysis |
construction of a reporter strain by inserting a gene fusion of apcA, the first gene of the apc-bal operon, with the gene for the fluorescent protein mCherry into the chromosome. mCherry production is proportional to the applied acetophenone concentrations. The reporter strain allows quantification of acetophenone within a concentration range of 50 microM (detection limit) to 250 microM after 12 and 24 h. Production of the Apc-mCherry fusion protein in the reporter strain is highly specific and responds to acetophenone and both enantiomers of 1-phenylethanol |
Aromatoleum aromaticum |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
6.4.1.8 |
Aromatoleum aromaticum |
Q5P5G2 and Q5P5G3 and Q5P5G4 and Q5P5G5 and Q5P5G6 |
Q5P5G2 i.e. alpha-subunit, Q5P5G3 i.e. beta-subunit, Q5P5G4 i.e. gamma-subunit, Q5P5G5 i.e. delta-subunit, Q5P5G6 i.e. epsilon-subunit |
- |
6.4.1.8 |
Aromatoleum aromaticum EbN1 |
Q5P5G2 and Q5P5G3 and Q5P5G4 and Q5P5G5 and Q5P5G6 |
Q5P5G2 i.e. alpha-subunit, Q5P5G3 i.e. beta-subunit, Q5P5G4 i.e. gamma-subunit, Q5P5G5 i.e. delta-subunit, Q5P5G6 i.e. epsilon-subunit |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
6.4.1.8 |
Apc1 |
alpha-subunit |
Aromatoleum aromaticum |
6.4.1.8 |
APC2 |
beta-subunit |
Aromatoleum aromaticum |
6.4.1.8 |
Apc3 |
gamma-subunit |
Aromatoleum aromaticum |
6.4.1.8 |
Apc4 |
delta-subunit |
Aromatoleum aromaticum |
6.4.1.8 |
Apc5 |
epsilon-subunit |
Aromatoleum aromaticum |
Expression
EC Number |
Organism |
Comment |
Expression |
---|
6.4.1.8 |
Aromatoleum aromaticum |
expression of alpha-subunit is proportional to the applied acetophenone concentrations |
up |