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Literature summary extracted from

  • Bruno, M.; Hofmann, M.; Vermathen, M.; Alder, A.; Beyer, P.; Al-Babili, S.
    On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7 (2014), FEBS Lett., 588, 1802-1807 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.68 gene ccd7, recombinant expression in Escherichia coli strain BL21(DE3) Pisum sativum
1.13.11.68 gene ccd7, recombinant expression in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.68 additional information
-
 additional information Michaelis-Menten kinetics Pisum sativum
1.13.11.68 additional information
-
 additional information Michaelis-Menten kinetics Arabidopsis thaliana
1.13.11.68 0.0411
-
 9-cis-beta-carotene recombinant enzyme, pH not specified in the publication, 28°C Arabidopsis thaliana
1.13.11.68 0.0596
-
 9-cis-beta-carotene recombinant enzyme, pH not specified in the publication, 28°C Pisum sativum
1.13.11.68 0.1487
-
 9-cis-zeaxanthin recombinant enzyme, pH not specified in the publication, 28°C Arabidopsis thaliana
1.13.11.68 0.626
-
 9-cis-zeaxanthin recombinant enzyme, pH not specified in the publication, 28°C Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.68 9-cis-beta-carotene + O2 Pisum sativum
-
 9-cis-10'-apo-beta-carotenal + beta-ionone
-
 ?
1.13.11.68 9-cis-beta-carotene + O2 Arabidopsis thaliana
-
 9-cis-10'-apo-beta-carotenal + beta-ionone
-
 ?
1.13.11.68 9-cis-zeaxanthin + O2 Pisum sativum
-
 9-cis-3-hydroxy-beta-apo-10'-carotenal + 3-hydroxy-beta-ionone
-
 ?
1.13.11.68 9-cis-zeaxanthin + O2 Arabidopsis thaliana
-
 9-cis-3-hydroxy-beta-apo-10'-carotenal + 3-hydroxy-beta-ionone
-
 ?
1.13.11.68 additional information Arabidopsis thaliana substrate- and stereospecificity of Arabidopsis thaliana carotenoid cleavage dioxygenase 7, overview ?
-
 ?
1.13.11.68 additional information Pisum sativum substrate- and stereospecificity of Pisum sativum carotenoid cleavage dioxygenase 7, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.68 Arabidopsis thaliana Q7XJM2
-
-
1.13.11.68 Pisum sativum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.68 9'-cis-cryptoxanthin + O2
-
 Arabidopsis thaliana ?
-
 ?
1.13.11.68 9-cis-beta-carotene + O2
-
 Pisum sativum 9-cis-10'-apo-beta-carotenal + beta-ionone
-
 ?
1.13.11.68 9-cis-beta-carotene + O2
-
 Arabidopsis thaliana 9-cis-10'-apo-beta-carotenal + beta-ionone
-
 ?
1.13.11.68 9-cis-cryptoxanthin + O2
-
 Arabidopsis thaliana ?
-
 ?
1.13.11.68 9-cis-lutein + O2
-
 Pisum sativum 9-cis-3-hydroxy-epsilon-apo-10'-carotenal + 3-hydroxy-beta-ionone
-
 ?
1.13.11.68 9-cis-lutein + O2
-
 Arabidopsis thaliana 9-cis-3-hydroxy-epsilon-apo-10'-carotenal + 3-hydroxy-beta-ionone
-
 ?
1.13.11.68 9-cis-zeaxanthin + O2
-
 Pisum sativum 9-cis-3-hydroxy-beta-apo-10'-carotenal + 3-hydroxy-beta-ionone
-
 ?
1.13.11.68 9-cis-zeaxanthin + O2
-
 Arabidopsis thaliana 9-cis-3-hydroxy-beta-apo-10'-carotenal + 3-hydroxy-beta-ionone
-
 ?
1.13.11.68 additional information substrate- and stereospecificity of Arabidopsis thaliana carotenoid cleavage dioxygenase 7, overview Arabidopsis thaliana ?
-
 ?
1.13.11.68 additional information substrate- and stereospecificity of Pisum sativum carotenoid cleavage dioxygenase 7, overview Pisum sativum ?
-
 ?
1.13.11.68 additional information enzyme CCD7 cleaves different 9-cis-carotenoids, yielding hydroxylated 9-cis-apo-10'-carotenals that may lead to hydroxylated carlactones, but show highest affinity for 9-cis-beta-carotene. The enzyme has a higher affinity to 9-cis-beta-carotene than to 9-cis-zeaxanthin, but converts the latter with higher rate. No detectable conversion of 9-cis-violaxanthin, all-trans-lutein, -zeaxanthin or -cryptoxanthin Pisum sativum ?
-
 ?
1.13.11.68 additional information enzyme CCD7 cleaves different 9-cis-carotenoids, yielding hydroxylated 9-cis-apo-10'-carotenals that may lead to hydroxylated carlactones, but show highest affinity for 9-cis-beta-carotene. The enzyme has a higher affinity to 9-cis-beta-carotene than to 9-cis-zeaxanthin, but converts the latter with higher rate. No detectable conversion of 9-cis-violaxanthin, all-trans-lutein, -zeaxanthin or -cryptoxanthin Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.68 carotenoid cleavage dioxygenase 7
-
 Pisum sativum
1.13.11.68 carotenoid cleavage dioxygenase 7
-
 Arabidopsis thaliana
1.13.11.68 CCD7
-
 Pisum sativum
1.13.11.68 CCD7
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.68 28
-
 assay at Pisum sativum
1.13.11.68 28
-
 assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.13.11.68 physiological function strigolactones are phytohormones synthesized from carotenoids via a stereospecific pathway involving the carotenoid cleavage dioxygenases 7 (CCD7) and 8. CCD7 cleaves 9-cis-beta-carotene to form a supposedly 9-cis-configured beta-apo-10'-carotenal Pisum sativum
1.13.11.68 physiological function strigolactones are phytohormones synthesized from carotenoids via a stereospecific pathway involving the carotenoid cleavage dioxygenases 7 (CCD7) and 8. CCD7 cleaves 9-cis-beta-carotene to form a supposedly 9-cis-configured beta-apo-10'-carotenal Arabidopsis thaliana