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Literature summary extracted from

  • Guranowski, A.; Miersch, O.; Staswick, P.; Suza, W.; Wasternack, C.
    Substrate specificity and products of side-reactions catalyzed by jasmonate amino acid synthetase (JAR1) (2007), FEBS Lett., 581, 815-820 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.52 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.52 Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.52 ATP + jasmonate + an L-amino acid Arabidopsis thaliana
-
 AMP + diphosphate + a jasmonoyl-L-amino acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.52 Arabidopsis thaliana
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.52 0.00024
-
 using (+)-jasmonate as substrate, at pH 8.6 and 25°C Arabidopsis thaliana
6.3.2.52 0.00756
-
 using 9,10-dihydro-jasmonate as substrate, at pH 8.6 and 25°C Arabidopsis thaliana
6.3.2.52 0.0105
-
 using (+/-)-jasmonate as substrate, at pH 8.6 and 25°C Arabidopsis thaliana
6.3.2.52 0.0298
-
 using (-)-jasmonate as substrate, at pH 8.6 and 25°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.52 ATP + (+)-jasmonate + L-Ile very low activity Arabidopsis thaliana AMP + diphosphate + jasmonoyl-L-Ile
-
 ?
6.3.2.52 ATP + (+/-)-jasmonate + L-Ile
-
 Arabidopsis thaliana AMP + diphosphate + jasmonoyl-L-Ile
-
 ?
6.3.2.52 ATP + (-)-jasmonate + L-Ile the rate of synthesis of (-)-jasmonate is about 100fold faster than for (+)-jasmonate. Diadenosine tetraphosphate cannot substitute ATP Arabidopsis thaliana AMP + diphosphate + jasmonoyl-L-Ile
-
 ?
6.3.2.52 ATP + 9,10-dihydro-jasmonate + L-Ile
-
 Arabidopsis thaliana ?
-
 ?
6.3.2.52 ATP + jasmonate + an L-amino acid
-
 Arabidopsis thaliana AMP + diphosphate + a jasmonoyl-L-amino acid
-
 ?
6.3.2.52 additional information the rate of conjugation with 12-hydroxy-jasmonate and 3-oxo-2-(2Z-pentenyl)cyclopentane-1-butyric acid is about 1-2% that for jasmonate. The enzyme shows no activity with (+/-)-12-oxo-phytodienoic acid, 3-oxo-2-(2Z-pentenyl)cyclopentane-1-hexanoate, and 3-oxo-2-(2Z-pentenyl)cyclopentane-1-octanoate Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.52 JAR1
-
 Arabidopsis thaliana
6.3.2.52 jasmonate:amino acid synthetase
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.52 ATP
-
 Arabidopsis thaliana