BRENDA - Enzyme Database

Three enzymatic activities catalyze the oxidation of sulfide to thiosulfate in mammalian and invertebrate mitochondria

Hildebrandt, T.; Grieshaber, M.; FEBS J. 275, 3352-3361 (2008)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.5.8
H2S
the enzyme activity decreases when the ambient sulfide concentration exceeds 0.3 mM
Rattus norvegicus
1.8.5.8
additional information
not inhibited by up to 2 mM H2S
Arenicola marina
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.5.8
0.00287
-
hydrogen sulfide
at pH 7.4 and 25°C
Rattus norvegicus
1.8.5.8
0.00994
-
hydrogen sulfide
at pH 7.4 and 25°C
Arenicola marina
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.5.8
mitochondrion
-
Arenicola marina
5739
-
1.8.5.8
mitochondrion
-
Rattus norvegicus
5739
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.5.8
hydrogen sulfide + glutathione + a quinone
Rattus norvegicus
-
S-sulfanylglutathione + a quinol
-
-
?
1.8.5.8
hydrogen sulfide + glutathione + a quinone
Arenicola marina
-
S-sulfanylglutathione + a quinol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.5.8
Arenicola marina
B0LD08
-
-
1.8.5.8
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.5.8
Resource Q column chromatography, Source S column chromatography, and Superdex 75 gel filtration
Arenicola marina
1.8.5.8
Resource Q column chromatography, Source S column chromatography, and Superdex 75 gel filtration
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.5.8
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.5.8
hydrogen sulfide + glutathione + a quinone
-
744869
Rattus norvegicus
S-sulfanylglutathione + a quinol
-
-
-
?
1.8.5.8
hydrogen sulfide + glutathione + a quinone
-
744869
Arenicola marina
S-sulfanylglutathione + a quinol
-
-
-
?
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.5.8
H2S
the enzyme activity decreases when the ambient sulfide concentration exceeds 0.3 mM
Rattus norvegicus
1.8.5.8
additional information
not inhibited by up to 2 mM H2S
Arenicola marina
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.5.8
0.00287
-
hydrogen sulfide
at pH 7.4 and 25°C
Rattus norvegicus
1.8.5.8
0.00994
-
hydrogen sulfide
at pH 7.4 and 25°C
Arenicola marina
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.5.8
mitochondrion
-
Arenicola marina
5739
-
1.8.5.8
mitochondrion
-
Rattus norvegicus
5739
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.5.8
hydrogen sulfide + glutathione + a quinone
Rattus norvegicus
-
S-sulfanylglutathione + a quinol
-
-
?
1.8.5.8
hydrogen sulfide + glutathione + a quinone
Arenicola marina
-
S-sulfanylglutathione + a quinol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.5.8
Resource Q column chromatography, Source S column chromatography, and Superdex 75 gel filtration
Arenicola marina
1.8.5.8
Resource Q column chromatography, Source S column chromatography, and Superdex 75 gel filtration
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.5.8
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.5.8
hydrogen sulfide + glutathione + a quinone
-
744869
Rattus norvegicus
S-sulfanylglutathione + a quinol
-
-
-
?
1.8.5.8
hydrogen sulfide + glutathione + a quinone
-
744869
Arenicola marina
S-sulfanylglutathione + a quinol
-
-
-
?