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Literature summary extracted from

  • Almeida, R.; Alqueres, S.; Larentis, A.; Rössle, S.; Cardoso, A.; Almeida, W.; Bisch, P.; Alves, T.; Martins, O.
    Cloning, expression, partial characterization and structural modeling of a novel esterase from Pyrococcus furiosus (2006), Enzyme Microb. Technol., 39, 1128-1136 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expressed in Escherichia coli BL21(DE3) Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 PMSF completely inhibited by 1 mM Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Pyrococcus furiosus Q8TZJ1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-methylumbelliferyl acetate + H2O
-
 Pyrococcus furiosus 4-methylumbelliferol + acetate
-
 ?
3.1.1.1 4-methylumbelliferyl heptanoate + H2O
-
 Pyrococcus furiosus 4-methylumbelliferol + heptanoate
-
 ?
3.1.1.1 4-methylumbelliferyl palmitate + H2O
-
 Pyrococcus furiosus 4-methylumbelliferol + palmitate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 PF2001
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 60
-
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 40 80 40°C: about 50% of maximal activity, 70°C: about 65% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 75
-
 thermostable for 120 min Pyrococcus furiosus
3.1.1.1 100
-
 10 min, complete loss of activity Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7
-
-
 Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 6 8 pH 6.0: 71% of maximal activity, pH 8.0: 82% of maximal activity Pyrococcus furiosus