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Literature summary extracted from

  • Punj, S.; John, G.
    Purification and identification of an FMN-dependent NAD(P)H azoreductase from Enterococcus faecalis (2009), Curr. Issues Mol. Biol., 11, 59-66 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.17 0.011
-
 4'-(dimethylamino)-azobenzene-2-carboxylic acid pH 7.2, 25°C Enterococcus faecalis
1.7.1.17 0.082
-
 NADH pH 7.2, 25°C Enterococcus faecalis
1.7.1.17 15
-
 NADPH pH 7.2, 25°C Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.17 Enterococcus faecalis
-
-
-
1.7.1.17 Enterococcus faecalis ATCC 27274
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.17 0.626
-
 cofactor NADPH, pH 7.0, 25°C Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Enterococcus faecalis 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+
-
 ?
1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Enterococcus faecalis ATCC 27274 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+
-
 ?
1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADPH + 2 H+ i.e. methyl red Enterococcus faecalis 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NADP+
-
 ?
1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADPH + 2 H+ i.e. methyl red Enterococcus faecalis ATCC 27274 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.17 FMN Km value 0.003 mM Enterococcus faecalis
1.7.1.17 NADH 180fold preferred over NADPH Enterococcus faecalis
1.7.1.17 NADPH NADH is 180fold preferred over NADPH Enterococcus faecalis