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Literature summary extracted from

  • Hu, Y.; Palmer, S.; Munoz, H.; Bullard, J.
    High throughput screen identifies natural product inhibitor of phenylalanyl-tRNA synthetase from Pseudomonas aeruginosa and Streptococcus pneumoniae (2015), Curr. Drug Discov. Technol., 11, 279-292 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.20 expressed in Escherichia coli Rosetta 2(DE3) cells Pseudomonas aeruginosa
6.1.1.20 expressed in Escherichia coli Rosetta 2(DE3) cells Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.20 1,1',6,6',7,7'-hexahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)[2,2'-binaphthalene]-8,8'-dicarbaldehyde
-
 Pseudomonas aeruginosa
6.1.1.20 1,1',6,6',7,7'-hexahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)[2,2'-binaphthalene]-8,8'-dicarbaldehyde
-
 Streptococcus pneumoniae
6.1.1.20 BPP_03B04
-
 Pseudomonas aeruginosa
6.1.1.20 BPP_03B04
-
 Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.20 0.00094
-
 tRNAPhe at pH 7.5 and 37°C Streptococcus pneumoniae
6.1.1.20 0.0012
-
 tRNAPhe at pH 7.5 and 37°C Pseudomonas aeruginosa
6.1.1.20 0.021
-
 L-phenylalanine at pH 7.5 and 37°C Streptococcus pneumoniae
6.1.1.20 0.048
-
 L-phenylalanine at pH 7.5 and 37°C Pseudomonas aeruginosa
6.1.1.20 0.2
-
 ATP at pH 7.5 and 37°C Pseudomonas aeruginosa
6.1.1.20 0.225
-
 ATP at pH 7.5 and 37°C Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe Pseudomonas aeruginosa
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 ATP + L-phenylalanine + tRNAPhe Streptococcus pneumoniae
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.20 Pseudomonas aeruginosa
-
-
-
6.1.1.20 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.20 Ni-NTA column chromatography Pseudomonas aeruginosa
6.1.1.20 Ni-NTA column chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
 Pseudomonas aeruginosa AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
 Streptococcus pneumoniae AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.20 heterodimer 1 * 80000 + 1 * 41000, SDS-PAGE Pseudomonas aeruginosa
6.1.1.20 heterodimer 1 * 85000 + 1 * 42000, SDS-PAGE Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
6.1.1.20 Phenylalanine-tRNA synthetase
-
 Pseudomonas aeruginosa
6.1.1.20 Phenylalanine-tRNA synthetase
-
 Streptococcus pneumoniae
6.1.1.20 PheRS
-
 Pseudomonas aeruginosa
6.1.1.20 PheRS
-
 Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.20 0.74
-
 tRNAPhe at pH 7.5 and 37°C Pseudomonas aeruginosa
6.1.1.20 0.93
-
 tRNAPhe at pH 7.5 and 37°C Streptococcus pneumoniae
6.1.1.20 13.2
-
 ATP at pH 7.5 and 37°C Pseudomonas aeruginosa
6.1.1.20 15.5
-
 ATP at pH 7.5 and 37°C Streptococcus pneumoniae
6.1.1.20 16.2
-
 L-phenylalanine at pH 7.5 and 37°C Pseudomonas aeruginosa
6.1.1.20 22.5
-
 L-phenylalanine at pH 7.5 and 37°C Streptococcus pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.20 ATP
-
 Pseudomonas aeruginosa
6.1.1.20 ATP
-
 Streptococcus pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.1.1.20 0.00083
-
 BPP_03B04 at pH 7.5 and 37°C Pseudomonas aeruginosa
6.1.1.20 0.00083
-
 1,1',6,6',7,7'-hexahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)[2,2'-binaphthalene]-8,8'-dicarbaldehyde at pH 7.5 and 37° Pseudomonas aeruginosa
6.1.1.20 0.00098
-
 BPP_03B04 at pH 7.5 and 37°C Streptococcus pneumoniae
6.1.1.20 0.00098
-
 1,1',6,6',7,7'-hexahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)[2,2'-binaphthalene]-8,8'-dicarbaldehyde at pH 7.5 and 37° Streptococcus pneumoniae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.1.1.20 0.0023
-
 at pH 7.5 and 37°C Pseudomonas aeruginosa BPP_03B04
6.1.1.20 0.0023
-
 at pH 7.5 and 37° Pseudomonas aeruginosa 1,1',6,6',7,7'-hexahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)[2,2'-binaphthalene]-8,8'-dicarbaldehyde
6.1.1.20 0.0049
-
 at pH 7.5 and 37°C Streptococcus pneumoniae BPP_03B04
6.1.1.20 0.0049
-
 at pH 7.5 and 37° Streptococcus pneumoniae 1,1',6,6',7,7'-hexahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)[2,2'-binaphthalene]-8,8'-dicarbaldehyde

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.20 64
-
 tRNAPhe at pH 7.5 and 37°C Pseudomonas aeruginosa
6.1.1.20 66
-
 ATP at pH 7.5 and 37°C Pseudomonas aeruginosa
6.1.1.20 70
-
 ATP at pH 7.5 and 37°C Streptococcus pneumoniae
6.1.1.20 340
-
 L-phenylalanine at pH 7.5 and 37°C Pseudomonas aeruginosa
6.1.1.20 1000
-
 tRNAPhe at pH 7.5 and 37°C Streptococcus pneumoniae
6.1.1.20 1100
-
 L-phenylalanine at pH 7.5 and 37°C Streptococcus pneumoniae