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Literature summary extracted from
- Fast purification method of functional LPMOs from Streptomyces ambofaciens by affinity adsorption (2017), Carbohydr. Res., 448, 205-211 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.99.53 | expression in Escherichia coli | Streptomyces ambofaciens |
| 1.14.99.54 | expression in Escherichia coli | Streptomyces ambofaciens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.99.53 | Streptomyces ambofaciens | - |
- |
- |
| 1.14.99.54 | Streptomyces ambofaciens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.99.53 | simple purification method by affinity adsorption to obtain functional lytic polysaccharide monooxygenases. Purification follows a binding-elution protocol with low-grade polysaccharides including Avicel | Streptomyces ambofaciens |
| 1.14.99.54 | simple purification method by affinity adsorption to obtain functional lytic polysaccharide monooxygenases. Purification follows a binding-elution protocol with low-grade polysaccharides including Avicel | Streptomyces ambofaciens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.53 | beta-chitin + ascorbic acid + O2 | - |
Streptomyces ambofaciens | ? + dehydroascorbate + H2O | - |
? |  |
| 1.14.99.54 | avicel + ascorbic acid + O2 | - |
Streptomyces ambofaciens | ? + dehydroascorbate + H2O | - |
? | |
| 1.14.99.54 | filter paper + ascorbic acid + O2 | - |
Streptomyces ambofaciens | ? + dehydroascorbate + H2O | - |
? | |
| 1.14.99.54 | additional information | products are oxidized cellooligosaccharides with DP of 4-8. No substrate: beta-chitin | Streptomyces ambofaciens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.99.53 | ? | x * 15029, calculated, x * 15000, SDS-PAGE | Streptomyces ambofaciens |
| 1.14.99.54 | ? | x * 34689, calculated, x * 35000, SDS-PAGE | Streptomyces ambofaciens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.99.53 | LPMO10B | - |
Streptomyces ambofaciens |
| 1.14.99.54 | LPMO10C | - |
Streptomyces ambofaciens |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.14.99.53 | Streptomyces ambofaciens | calculated | - |
4.8 |
| 1.14.99.54 | Streptomyces ambofaciens | calculated | - |
5 |
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