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Literature summary extracted from

  • Park, Y.; Yeo, H.; Lee, J.
    Structural analysis of the NH3-dependent NAD+ synthetase from Deinococcus radiodurans (2014), Bull. Korean Chem. Soc., 35, 2863-2866 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.5 gene nadE, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) star pLysS Deinococcus radiodurans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.1.5 purified recombinant detagged enzyme, sitting drop vapor diffusion method, mixing of 30 mg/ml protein in 0.2 M NaCl, 20 mM Tris-HCl, pH 8.0, 1 mM DTT, 5 mM MgCl2, and 5% glycerol, with reservoir solution containing 20% PEG 4000, 0.2 M lithium sulfate, 0.1 M MES, pH 6.0, 23°C, 3 days, method optimization, X-ray diffraction structure determination and analysis at 2.60 A resolution, molecular replacement Deinococcus radiodurans

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.5 Deinococcus radiodurans Q9RYV5
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6.3.1.5 Deinococcus radiodurans ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422 Q9RYV5
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-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.5 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) star pLysS by nickel affinity chromatography, tag cleavage by a protease, another step of nickel affinity chromatography, and gel filtration Deinococcus radiodurans

Subunits

EC Number Subunits Comment Organism
6.3.1.5 More enzyme structure analysis, overview Deinococcus radiodurans

Synonyms

EC Number Synonyms Comment Organism
6.3.1.5 draNADS
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 Deinococcus radiodurans
6.3.1.5 NadE
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 Deinococcus radiodurans
6.3.1.5 NH3-dependent NAD+ synthetase
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 Deinococcus radiodurans