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Literature summary extracted from
- Substrate affinity and catalytic efficiency are improved by decreasing glycosylation sites in Trichoderma reesei cellobiohydrolase I expressed in Pichia pastoris (2016), Biotechnol. Lett., 38, 483-488 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.176 | expressed in Pichia pastoris Pichia Pink strain2 | Trichoderma reesei |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.176 | N45A | the mutant shows 114% of wild type activity | Trichoderma reesei |
| 3.2.1.176 | N45A/N64A | the mutant shows 143% of wild type activity | Trichoderma reesei |
| 3.2.1.176 | N64A | the mutant shows 106% of wild type activity | Trichoderma reesei |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.176 | cellulose + H2O | Trichoderma reesei | - |
cellobiose + ? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.176 | Trichoderma reesei | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.176 | glycoprotein | - |
Trichoderma reesei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.176 | carboxymethyl cellulose + H2O | - |
Trichoderma reesei | ? | - |
? | |
| 3.2.1.176 | cellulose + H2O | - |
Trichoderma reesei | cellobiose + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.176 | ? | x * 100000, glycosylated recombinant enzyme, SDS-PAGE | Trichoderma reesei |
| 3.2.1.176 | ? | x * 65000, deglycosylated recombinant enzyme, SDS-PAGE | Trichoderma reesei |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.176 | CBH I | - |
Trichoderma reesei |
| 3.2.1.176 | Cel7A | - |
Trichoderma reesei |
| 3.2.1.176 | cellobiohydrolase I | - |
Trichoderma reesei |
| 3.2.1.176 | exoglucanase | - |
Trichoderma reesei |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.176 | 70 | - |
- |
Trichoderma reesei |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.176 | 5 | - |
- |
Trichoderma reesei |
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Release 2023.1 (January 2023)
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