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Literature summary extracted from
- Identification and characterization of a novel bifunctional DELTA12/DELTA15-fatty acid desaturase gene from Rhodosporidium kratochvilovae (2016), Biotechnol. Lett., 38, 1155-1164 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.19.13 | gene RKD12, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae | Rhodotorula kratochvilovae |
| 1.14.19.45 | gene RKD12, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae | Rhodotorula kratochvilovae |
| 1.14.19.45 | RKD12 is further transformed into Saccharomyces cerevisiae for functional characterization | Rhodotorula kratochvilovae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.13 | additional information | Rhodotorula kratochvilovae | the enzyme produces linoleic acid and alpha-linolenic acid, overview | ? | - |
? |  |
| 1.14.19.13 | additional information | Rhodotorula kratochvilovae YM25235 | the enzyme produces linoleic acid and alpha-linolenic acid, overview | ? | - |
? | |
| 1.14.19.45 | a 1-oleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Rhodotorula kratochvilovae | - |
a 1-linoleoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
| 1.14.19.45 | additional information | Rhodotorula kratochvilovae | the enzyme produces linoleic acid and alpha-linolenic acid, overview | ? | - |
? | |
| 1.14.19.45 | additional information | Rhodotorula kratochvilovae YM25235 | the enzyme produces linoleic acid and alpha-linolenic acid, overview | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.13 | Rhodotorula kratochvilovae | A0A024B710 | isolated from Chenghai Lake in Yunnan, China | - |
| 1.14.19.13 | Rhodotorula kratochvilovae YM25235 | A0A024B710 | isolated from Chenghai Lake in Yunnan, China | - |
| 1.14.19.45 | Rhodotorula kratochvilovae | A0A024B710 | - |
- |
| 1.14.19.45 | Rhodotorula kratochvilovae | A0A024B710 | isolated from Chenghai Lake in Yunnan, China | - |
| 1.14.19.45 | Rhodotorula kratochvilovae YM25235 | A0A024B710 | - |
- |
| 1.14.19.45 | Rhodotorula kratochvilovae YM25235 | A0A024B710 | isolated from Chenghai Lake in Yunnan, China | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.13 | (9Z,12Z)-hexadeca-9,12-dienoyl-CoA + reduced acceptor + O2 | - |
Rhodotorula kratochvilovae | (9Z,12Z,15Z)-hexadeca-9,12,15-trienoyl-CoA + acceptor + 2 H2O | - |
? | |
| 1.14.19.13 | (9Z,12Z)-hexadeca-9,12-dienoyl-CoA + reduced acceptor + O2 | - |
Rhodotorula kratochvilovae YM25235 | (9Z,12Z,15Z)-hexadeca-9,12,15-trienoyl-CoA + acceptor + 2 H2O | - |
? | |
| 1.14.19.13 | additional information | the enzyme produces linoleic acid and alpha-linolenic acid, overview | Rhodotorula kratochvilovae | ? | - |
? | |
| 1.14.19.13 | additional information | absence of desaturation activity of RKD12 for C16 substrates | Rhodotorula kratochvilovae | ? | - |
? | |
| 1.14.19.13 | additional information | the enzyme produces linoleic acid and alpha-linolenic acid, overview | Rhodotorula kratochvilovae YM25235 | ? | - |
? | |
| 1.14.19.13 | additional information | absence of desaturation activity of RKD12 for C16 substrates | Rhodotorula kratochvilovae YM25235 | ? | - |
? | |
| 1.14.19.45 | a 1-oleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | - |
Rhodotorula kratochvilovae | a 1-linoleoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
| 1.14.19.45 | additional information | the enzyme produces linoleic acid and alpha-linolenic acid, overview | Rhodotorula kratochvilovae | ? | - |
? | |
| 1.14.19.45 | additional information | absence of desaturation activity of RKD12 for C16 substrates | Rhodotorula kratochvilovae | ? | - |
? | |
| 1.14.19.45 | additional information | the enzyme produces linoleic acid and alpha-linolenic acid, overview | Rhodotorula kratochvilovae YM25235 | ? | - |
? | |
| 1.14.19.45 | additional information | absence of desaturation activity of RKD12 for C16 substrates | Rhodotorula kratochvilovae YM25235 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.19.13 | ? | x * 50600, about, sequence calculation | Rhodotorula kratochvilovae |
| 1.14.19.45 | ? | x * 50600, about, sequence calculation | Rhodotorula kratochvilovae |
| 1.14.19.45 | ? | x * 50634, calculated from sequence | Rhodotorula kratochvilovae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.19.13 | bifunctional DELTA12/DELTA15-fatty acid desaturase | - |
Rhodotorula kratochvilovae |
| 1.14.19.13 | DELTA12-desaturase | - |
Rhodotorula kratochvilovae |
| 1.14.19.13 | More | cf. EC 1.14.19.45 | Rhodotorula kratochvilovae |
| 1.14.19.13 | RKD12 | - |
Rhodotorula kratochvilovae |
| 1.14.19.45 | bifunctional DELTA12/DELTA15-fatty acid desaturase | - |
Rhodotorula kratochvilovae |
| 1.14.19.45 | DELTA(12) fatty acid desaturase | - |
Rhodotorula kratochvilovae |
| 1.14.19.45 | DELTA12-desaturase | - |
Rhodotorula kratochvilovae |
| 1.14.19.45 | More | cf. EC 1.14.19.13 | Rhodotorula kratochvilovae |
| 1.14.19.45 | RKD12 | - |
Rhodotorula kratochvilovae |
| 1.14.19.45 | RKD12 | bifunctional DELTA12/DELTA15-desaturase gene | Rhodotorula kratochvilovae |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.14.19.13 | Rhodotorula kratochvilovae | a culture temperature downshift from 28°C to 15°C induces the enzyme | up |
| 1.14.19.45 | Rhodotorula kratochvilovae | a culture temperature downshift from 28°C to 15°C induces the enzyme | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.19.13 | evolution | bifunctional DELTA12/DELTA15-desaturases are widely found in fungi species belonging to Ascomycota and Basidiomycota. Enzyme RKD12 shares higher homology to the Cop-odeA, it only exhibits DELTA12 and DELTA15 desaturation activity on C18 substrates without preference | Rhodotorula kratochvilovae |
| 1.14.19.13 | physiological function | for better adaptation to low temperatures, Rhodosporidium kratochvilovae YM25235 may have evolved some adaptative mechanisms that might include the alteration of unsaturation levels of fatty acids in membrane lipids involving the enzyme | Rhodotorula kratochvilovae |
| 1.14.19.45 | evolution | bifunctional DELTA12/DELTA15-desaturases are widely found in fungi species belonging to Ascomycota and Basidiomycota. Enzyme RKD12 shares higher homology to the Cop-odeA, it only exhibits DELTA12 and DELTA15 desaturation activity on C18 substrates without preference | Rhodotorula kratochvilovae |
| 1.14.19.45 | physiological function | for better adaptation to low temperatures, YM25235 may have evolved some adaptative mechanisms that might include the alteration of unsaturation levels of fatty acids in membrane lipids involving th enzyme | Rhodotorula kratochvilovae |
| 1.14.19.45 | physiological function | increased RKD12 mRNA expression level and PUFAs content at low temperature might be helpful for the cold adaptation of Rhodosporidium kratochvilovae YM25235 | Rhodotorula kratochvilovae |
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