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Literature summary extracted from

  • Orellana, C.A.; Marcellin, E.; Gray, P.P.; Nielsen, L.K.
    Overexpression of the regulatory subunit of glutamate-cysteine ligase enhances monoclonal antibody production in CHO cells (2017), Biotechnol. Bioeng., 114, 1825-1836 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.2 genes I79_010621 or Gclc and I79_022778 or Gclm, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative RT-PCR expression analysis of recombinant and wild-type enzyme subunits, determination of isozyme Gclc-X2 lacking the first 5 amino acids compared to isozymes Gclc-X0 and Gclc-X1, recombinant overexpression of the enzyme subunits in a CHO-T cell line Epi-CHO, derived from CHO-K1 cells. Gclc-X2 but not Gclm overexpression increases Gcl activity Cricetulus griseus

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.2 additional information overexpression of subunit Gclc-X2 leads to upregulation of the proteins Abcf1, Fkbp4, and Eif3h, as well as to downregulation of protein Lamb1. There is no significant difference in growth rate during exponential phase at 32C between Gclm+ or Gclc-X2+ populations and the wild-type population. A higher cell proliferation observed in the Gclc overexpressing population Cricetulus griseus

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Cricetulus griseus G3HIY9 AND G3IG96 genes I79_010621 and I79_022778 encoding catalytic and regulatory subunits
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Synonyms

EC Number Synonyms Comment Organism
6.3.2.2 GCL
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 Cricetulus griseus
6.3.2.2 GCLC
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 Cricetulus griseus
6.3.2.2 Gclc-X2
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 Cricetulus griseus
6.3.2.2 GCLM
-
 Cricetulus griseus
6.3.2.2 I79_022778
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 Cricetulus griseus

General Information

EC Number General Information Comment Organism
6.3.2.2 malfunction overexpression of subunit Gclc-X2 leads to upregulation of the proteins Abcf1, Fkbp4, and Eif3h, as well as to downregulation of protein Lamb1. There is no significant difference in growth rate during exponential phase at 32C between Gclm+ or Gclc-X2+ populations and the wild-type population. A higher cell proliferation observed in the Gclc overexpressing population. Gclc-X2 but not Gclm overexpression increases Gcl activity Cricetulus griseus
6.3.2.2 metabolism the enzyme catalyzes the first step in the glutathione biosynthesis Cricetulus griseus