Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rashamuse, K.; Sanyika Tendai, W.; Mathiba, K.; Ngcobo, T.; Mtimka, S.; Brady, D.
    Metagenomic mining of glycoside hydrolases from the hindgut bacterial symbionts of a termite (Trinervitermes trinervoides) and the characterization of a multimodular beta-1,4-xylanase (GH11) (2017), Biotechnol. Appl. Biochem., 64, 174-186 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.8 additional information construction of truncated mutants, lacking the carbohydrate binding module CBM, which functions to selectively bind insoluble xylan and increase the rate of hydrolysis. Xyl1A corresponds to the GH11 domain coding sequence, Xyl1B corresponds to the GH11 and the CBM1 domain coding sequences, and Xyl1C corresponds to the two CBM coding sequences uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 beechwood xylan Km value 1.2 mg/ml for wild-type, 2.8 mg/ml for mutant XylA, 1.9 mg/ml for mutant XylB, pH 6.0, 50°C uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 uncultured bacterium A0A127JBT0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beechwood xylan + H2O
-
 uncultured bacterium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 55000, wild-type, x * 28000, mutant XylA, x * 40000, mutant XylB, x * 26000, mutant XylC, SDS-PAGE uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50
-
-
 uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 30 60 more than 60% of maximum activity uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 38.9
-
 beechwood xylan mutant XylA, pH 6.0, 50°C uncultured bacterium
3.2.1.8 49.6
-
 beechwood xylan mutant XylB, pH 6.0, 50°C uncultured bacterium
3.2.1.8 55.3
-
 beechwood xylan wild-type, pH 6.0, 50°C uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6
-
-
 uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 5 8 more than 80% of maximum activity uncultured bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 beechwood xylan kcat/Km value 47 ml/mg/s for wild-type, 13.9 ml/mg/s for mutant XylA, 26.1 ml/mg/s for mutant XylB, pH 6.0, 50°C uncultured bacterium