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Literature summary extracted from

  • Gao, Z.
    Purification and characterization of a novel lichenase from Bacillus licheniformis GZ-2 (2016), Biotechnol. Appl. Biochem., 63, 249-256 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.73 2-mercaptoethanol 1 mM, 115% of initial activity Bacillus licheniformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.73 expression in Escherichia coli Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.73 EDTA 1 mM, 77% residual activity Bacillus licheniformis
3.2.1.73 Fe3+ 1 mM, 65% residual activity Bacillus licheniformis
3.2.1.73 hexadecyltrimethylammonium bromide 1 mM, no residual activity Bacillus licheniformis
3.2.1.73 Mn2+ 1 mM, 87% residual activity Bacillus licheniformis
3.2.1.73 N-bromosuccinimide 1 mM, no residual activity Bacillus licheniformis
3.2.1.73 SDS 1 mM, no residual activity Bacillus licheniformis
3.2.1.73 Zn2+ 1 mM, 87% residual activity Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.73 additional information
-
 lichenan Km value 5.11 mg/ml, pH 6.5, 60°C Bacillus licheniformis
3.2.1.73 additional information
-
 Barley beta-glucan Km value 7.42 mg/ml, pH 6.5, 60°C Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.73 Ca2+ 1 mM, 111% of initial activity Bacillus licheniformis
3.2.1.73 Co2+ 1 mM, 116% of initial activity Bacillus licheniformis
3.2.1.73 Ni2+ 1 mM, 114% of initial activity Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Bacillus licheniformis
-
-
-
3.2.1.73 Bacillus licheniformis GZ-2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73
-
 Bacillus licheniformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 8231
-
 pH 6.5, 60°C Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 barley beta-glucan + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.73 barley beta-glucan + H2O
-
 Bacillus licheniformis GZ-2 ?
-
 ?
3.2.1.73 lichenan + H2O
-
 Bacillus licheniformis cellotriose + cellotetraose + ?
-
 ?
3.2.1.73 lichenan + H2O
-
 Bacillus licheniformis GZ-2 cellotriose + cellotetraose + ?
-
 ?
3.2.1.73 additional information enzyme exhibits strict specificity for beta-1,3-1,4-D-glucans Bacillus licheniformis ?
-
 ?
3.2.1.73 additional information enzyme exhibits strict specificity for beta-1,3-1,4-D-glucans Bacillus licheniformis GZ-2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.73 ? x * 25000, SDS-PAGE, x * 24400, calculated Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 60
-
-
 Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 6.5
-
-
 Bacillus licheniformis