BRENDA - Enzyme Database

Cloning and characterization of a novel beta-carotene hydroxylase gene from Lycium barbarum and its expression in Escherichia coli

Wu, J.; Ji, J.; Wang, G.; Li, Z.; Diao, J.; Wu, G.; Biotechnol. Appl. Biochem. 61, 637-645 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.15.24
expressed in Escherichia coli BL21(DE3) cells
Lycium barbarum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.15.24
chloroplast
-
Lycium barbarum
9507
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Lycium barbarum
-
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.24
Lycium barbarum
U5Y6R4
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.15.24
Ni-NTA column chromatography
Lycium barbarum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.15.24
flower
-
Lycium barbarum
-
1.14.15.24
fruit
highest expression
Lycium barbarum
-
1.14.15.24
leaf
-
Lycium barbarum
-
1.14.15.24
root
-
Lycium barbarum
-
1.14.15.24
stem
lowest expression
Lycium barbarum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
744536
Lycium barbarum
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.15.24
?
x * 34800, calculated from amino acid sequence; x * 37000, SDS-PAGE
Lycium barbarum
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.14.15.24
Lycium barbarum
calculated from amino acid sequence
-
8.4
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.24
expressed in Escherichia coli BL21(DE3) cells
Lycium barbarum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.15.24
chloroplast
-
Lycium barbarum
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Lycium barbarum
-
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.24
Ni-NTA column chromatography
Lycium barbarum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.15.24
flower
-
Lycium barbarum
-
1.14.15.24
fruit
highest expression
Lycium barbarum
-
1.14.15.24
leaf
-
Lycium barbarum
-
1.14.15.24
root
-
Lycium barbarum
-
1.14.15.24
stem
lowest expression
Lycium barbarum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
744536
Lycium barbarum
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.15.24
?
x * 34800, calculated from amino acid sequence; x * 37000, SDS-PAGE
Lycium barbarum
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.14.15.24
Lycium barbarum
calculated from amino acid sequence
-
8.4