EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | 2-Methylresorcinol | acts as enzyme substrate and inhibitor | Agaricus bisporus | |
1.14.18.1 | 4-ethylresorcinol | acts as enzyme substrate and inhibitor | Agaricus bisporus | |
1.14.18.1 | 4-methylresorcinol | acts as enzyme substrate and inhibitor | Agaricus bisporus | |
1.14.18.1 | additional information | structural mechanism of resorcinol inhibitors, overview | Agaricus bisporus | |
1.14.18.1 | resorcinol | acts as enzyme substrate and inhibitor | Agaricus bisporus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.18.1 | additional information | - |
additional information | Michaelis-Menten kinetics | Agaricus bisporus | |
1.14.18.1 | 0.08 | - |
4-methoxyphenol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 0.117 | - |
4-ethylresorcinol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 0.17 | - |
4-ethoxyphenol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 0.203 | - |
4-methylresorcinol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 0.21 | - |
L-tyrosine | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 0.46 | - |
resorcinol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 0.7 | - |
phenol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 0.92 | - |
hydroquinone | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 2.7 | - |
2-Methylresorcinol | pH 7.0, 25°C | Agaricus bisporus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | Cu2+ | a copper-containing enzyme | Agaricus bisporus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | 2 L-dopa + O2 | Agaricus bisporus | - |
2 dopaquinone + 2 H2O | - |
? | |
1.14.18.1 | L-tyrosine + O2 | Agaricus bisporus | - |
dopaquinone + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.18.1 | Agaricus bisporus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.18.1 | commercial preparation | - |
Agaricus bisporus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | 2 L-dopa + O2 | - |
Agaricus bisporus | 2 dopaquinone + 2 H2O | - |
? | |
1.14.18.1 | 2-methylresorcinol + O2 | acts as enzyme substrate and inhibitor, low activity | Agaricus bisporus | ? | - |
? | |
1.14.18.1 | 4-ethoxyphenol + O2 | - |
Agaricus bisporus | ? | - |
? | |
1.14.18.1 | 4-ethylresorcinol + O2 | acts as enzyme substrate and inhibitor | Agaricus bisporus | ? | - |
? | |
1.14.18.1 | 4-methoxyphenol + O2 | - |
Agaricus bisporus | ? | - |
? | |
1.14.18.1 | 4-methylresorcinol + O2 | acts as enzyme substrate and inhibitor | Agaricus bisporus | ? | - |
? | |
1.14.18.1 | hydroquinone + O2 | - |
Agaricus bisporus | ? | - |
? | |
1.14.18.1 | L-tyrosine + O2 | - |
Agaricus bisporus | dopaquinone + H2O | - |
? | |
1.14.18.1 | additional information | resorcinol and some derivatives, 4-ethylresorcinol, 2-methylresorcinol and 4-methylresorcinol, all act as substrates of tyrosinase if the catalytic cycle is completed with a reductant such as ascorbic acid or an o-diphenol such as 4-tert-butylcatechol. The reaction can also be carried out, adding hydrogen peroxide to the reaction medium. Measurement of the activity of the enzyme after pre-incubation with resorcinol, 4-ethylresorcinol or 4-methylresorcinol points to an apparent loss of activity at short times. If the measurements are extended over longer times, a burst is observed and the enzymatic activity is recovered, demonstrating that these compounds are not suicide substrates of the enzyme. These effects are not observed with 2-methylresorcinol. The docking results indicate that the binding of met-tyrosinase with these resorcinols occurs in the same way, but not with 2-methylresorcinol, due to steric hindrance. The enzyme assays are performed in preenceof ascorbic acid or hydrogen peroxide. Molecular docking simulations and modeling, overview | Agaricus bisporus | ? | - |
? | |
1.14.18.1 | phenol + O2 | - |
Agaricus bisporus | ? | - |
? | |
1.14.18.1 | resorcinol + O2 | acts as enzyme substrate and inhibitor | Agaricus bisporus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.18.1 | mushroom tyrosinase | - |
Agaricus bisporus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 25 | - |
assay at | Agaricus bisporus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.18.1 | 6 | - |
4-ethylresorcinol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 7.73 | - |
4-methylresorcinol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 7.9 | - |
L-tyrosine | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 9.94 | - |
2-Methylresorcinol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 12.7 | - |
phenol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 18.34 | - |
resorcinol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 101.5 | - |
hydroquinone | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 132 | - |
4-ethoxyphenol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 184.2 | - |
4-methoxyphenol | pH 7.0, 25°C | Agaricus bisporus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 7 | - |
assay at | Agaricus bisporus |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.14.18.1 | 0.0011 | - |
pH 7.0, 25°C, inhibition of the diphenolase activity of mushroom tyrosinase | Agaricus bisporus | 4-methylresorcinol | |
1.14.18.1 | 0.0019 | - |
pH 7.0, 25°C, inhibition of the monophenolase activity of mushroom tyrosinase | Agaricus bisporus | 4-ethylresorcinol | |
1.14.18.1 | 0.0033 | - |
pH 7.0, 25°C, inhibition of the diphenolase activity of mushroom tyrosinase | Agaricus bisporus | 4-ethylresorcinol | |
1.14.18.1 | 0.00931 | - |
pH 7.0, 25°C, inhibition of the monophenolase activity of mushroom tyrosinase | Agaricus bisporus | 4-ethylresorcinol | |
1.14.18.1 | 0.652 | - |
pH 7.0, 25°C, inhibition of the diphenolase activity of mushroom tyrosinase | Agaricus bisporus | resorcinol | |
1.14.18.1 | 0.717 | - |
pH 7.0, 25°C, inhibition of the diphenolase activity of mushroom tyrosinase | Agaricus bisporus | 2-Methylresorcinol | |
1.14.18.1 | 1.125 | - |
pH 7.0, 25°C, inhibition of the monophenolase activity of mushroom tyrosinase | Agaricus bisporus | resorcinol | |
1.14.18.1 | 1.415 | - |
pH 7.0, 25°C, inhibition of the monophenolase activity of mushroom tyrosinase | Agaricus bisporus | 2-Methylresorcinol |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.18.1 | 3.7 | - |
2-Methylresorcinol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 18.1 | - |
phenol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 37.6 | - |
L-tyrosine | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 38.1 | - |
4-methylresorcinol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 39.9 | - |
resorcinol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 51.3 | - |
4-ethylresorcinol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 110.3 | - |
hydroquinone | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 776.5 | - |
4-ethoxyphenol | pH 7.0, 25°C | Agaricus bisporus | |
1.14.18.1 | 2300 | - |
4-methoxyphenol | pH 7.0, 25°C | Agaricus bisporus |