EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.13.11.66 | purified enzyme free or in complex with substrate methylhydroquinone under anaerobic conditions, or with inhibitors 4-hydroxybenzoate and 4-nitrophenol, sitting drop vapor diffusion method, mixing of 0.001 ml of 8 mg/ml protein in 25 mM Tris, pH 7.0, 5 mM NaCl, and 0.5 mM ligand, with 0.001 ml of reservoir solution containing 14% PEG 3350, 0.35 M MgCl2, and 0.1 M MES, pH 6.5, 4°C, 1 day, the structure of the free enzyme is obtained by soaking the crystals in a stabilizing solution containing 16% PEG 3350, 0.35 M MgCl2, and 0.1 M MES, pH 6.5, for two days, changing the solution three times, in order to remove the ligands, X-ray diffraction structure determination and analysis at 1.90-2.40 resolution, molecular replacement using the coordinates of PnpCD structure from Pseudomonas sp. strain WBC-3, PDB ID 4ZXA as template, modeling | Sphingomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.66 | 4-hydroxybenzoate | binding structure, overview | Sphingomonas sp. | |
1.13.11.66 | 4-nitrophenol | binding structure, overview | Sphingomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.66 | Fe2+ | required for catalysis, each of the four beta subunits in the asymmetric unit of the enzyme crystal binds one Fe(II) ion. The iron ion in each beta subunit is coordinated to three protein residues, His258, Glu264, and His305 and a water molecule | Sphingomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.66 | benzene-1,4-diol + O2 | Sphingomonas sp. | - |
(2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
1.13.11.66 | benzene-1,4-diol + O2 | Sphingomonas sp. TTNP3 | - |
(2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.66 | Sphingomonas sp. | F8TW82 AND F8TW83 | alpha and beta subunits | - |
1.13.11.66 | Sphingomonas sp. TTNP3 | F8TW82 AND F8TW83 | alpha and beta subunits | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.13.11.66 | benzene-1,4-diol + O2 = (2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate | catalytic mechanism, overview | Sphingomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.66 | benzene-1,4-diol + O2 | - |
Sphingomonas sp. | (2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
1.13.11.66 | benzene-1,4-diol + O2 | - |
Sphingomonas sp. TTNP3 | (2Z,4E)-4-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
1.13.11.66 | methylhydroquinone + O2 | substrate binding structure, overview | Sphingomonas sp. | ? | - |
? | |
1.13.11.66 | methylhydroquinone + O2 | substrate binding structure, overview | Sphingomonas sp. TTNP3 | ? | - |
? | |
1.13.11.66 | additional information | hydroquinone 1,2-dioxygenase, a Fe(II) ring cleaving dioxygenase from Sphingomonas sp. strain TTNP3, oxidizes a wide range of hydroquinones to the corresponding 4-hydroxymuconic semialdehydes | Sphingomonas sp. | ? | - |
? | |
1.13.11.66 | additional information | hydroquinone 1,2-dioxygenase, a Fe(II) ring cleaving dioxygenase from Sphingomonas sp. strain TTNP3, oxidizes a wide range of hydroquinones to the corresponding 4-hydroxymuconic semialdehydes | Sphingomonas sp. TTNP3 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.66 | heterotetramer | 2 * 19000, subunit alpha, + 2 * 38000, subunit beta | Sphingomonas sp. |
1.13.11.66 | More | both subunits alpha and beta fold as a cupin, but that of the small alpha subunit lacks a competent metal binding pocket. Two tetramers are present in the asymmetric unit of the enzyme crystals | Sphingomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.66 | HQDO | - |
Sphingomonas sp. |
1.13.11.66 | type II HQDO | - |
Sphingomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.66 | 25 | - |
assay at | Sphingomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.66 | 7 | - |
assay at | Sphingomonas sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.11.66 | additional information | active site structure analysis, apo or with bound substrate methylhydroquinone, overview | Sphingomonas sp. |