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Literature summary extracted from

  • Englert, M.; Vargas-Rodriguez, O.; Reynolds, N.M.; Wang, Y.S.; Soell, D.; Umehara, T.
    A genomically modified Escherichia coli strain carrying an orthogonal E. coli histidyl-tRNA synthetase tRNAHis pair (2017), Biochim. Biophys. Acta, 1861, 3009-3015 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.21 expressed in Escherichia coli MEOV1 cells Caulobacter vibrioides

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.21 E131A inactive Caulobacter vibrioides
6.1.1.21 Y264A inactive Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis Caulobacter vibrioides
-
 AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.21 Caulobacter vibrioides
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.21 ATP + 1-methyl-L-histidine + tRNAHis
-
 Caulobacter vibrioides AMP + diphosphate + 1-methyl-L-histidyl-tRNAHis
-
 ?
6.1.1.21 ATP + 2-thio-L-histidine + tRNAHis low activity Caulobacter vibrioides AMP + diphosphate + 2-thio-L-histidyl-tRNAHis
-
 ?
6.1.1.21 ATP + 3-methyl-L-histidine + tRNAHis
-
 Caulobacter vibrioides AMP + diphosphate + 3-methyl-L-histidyl-tRNAHis
-
 ?
6.1.1.21 ATP + D-histidine + tRNAHis
-
 Caulobacter vibrioides AMP + diphosphate + D-histidyl-tRNAHis
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Caulobacter vibrioides AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis best substrate Caulobacter vibrioides AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
6.1.1.21 additional information no activity with DL-alpha-methyl-histidine, benzyl-L-histidine, 3-[4-thiazolyl]-L-alanine, 2-[thiazolyl]-L-alanine, 1,2,4-triazole-alanine, 2-furyl-L-alanine, 3-cyclopentane-L-alanine, and [3-thienyl]-DL-alanine Caulobacter vibrioides ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.21 HisRS
-
 Caulobacter vibrioides
6.1.1.21 Histidyl-tRNA synthetase
-
 Caulobacter vibrioides

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.21 ATP
-
 Caulobacter vibrioides