Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cabeza, M.; Guerrero, S.; Iglesias, A.; Arias, D.
    New enzymatic pathways for the reduction of reactive oxygen species in Entamoeba histolytica (2015), Biochim. Biophys. Acta, 1850, 1233-1244 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.4 expressed in Escherichia coli BL21(DE3) cells Entamoeba histolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.1.4 additional information no substrate inhibition at high NAD(P)H (up to 0.3 mM) Entamoeba histolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.4 0.0028
-
 oxidized Clostridium pasteurianum rubredoxin with NADPH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
1.18.1.4 0.0032
-
 oxidized Pyrococcus furiosus rubredoxin with NADPH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
1.18.1.4 0.0043
-
 oxidized Clostridium pasteurianum rubredoxin with NADH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
1.18.1.4 0.0052
-
 oxidized Pyrococcus furiosus rubredoxin with NADH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.4 41000
-
 gel filtration Entamoeba histolytica

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.4 Entamoeba histolytica
-
-
-
1.18.1.4 Entamoeba histolytica HM1-IMSS
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.4 HiTrap IMAC-Ni2+ column chromatography Entamoeba histolytica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.18.1.4 trophozoite
-
 Entamoeba histolytica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.4 additional information the enzyme has no activity with ferredoxins, flavodoxins, and thioredoxins Entamoeba histolytica ?
-
 ?
1.18.1.4 additional information the enzyme has no activity with ferredoxins, flavodoxins, and thioredoxins Entamoeba histolytica HM1-IMSS ?
-
 ?
1.18.1.4 oxidized Clostridium pasteurianum rubredoxin + NADH
-
 Entamoeba histolytica reduced Clostridium pasteurianum rubredoxin + NAD+ + H+
-
 ?
1.18.1.4 oxidized Clostridium pasteurianum rubredoxin + NADH
-
 Entamoeba histolytica HM1-IMSS reduced Clostridium pasteurianum rubredoxin + NAD+ + H+
-
 ?
1.18.1.4 oxidized Clostridium pasteurianum rubredoxin + NADPH
-
 Entamoeba histolytica reduced Clostridium pasteurianum rubredoxin + NADP+ + H+
-
 ?
1.18.1.4 oxidized Clostridium pasteurianum rubredoxin + NADPH
-
 Entamoeba histolytica HM1-IMSS reduced Clostridium pasteurianum rubredoxin + NADP+ + H+
-
 ?
1.18.1.4 oxidized flavodiiron protein + NADH
-
 Entamoeba histolytica reduced flavodiiron protein + NAD+ + H+
-
 ?
1.18.1.4 oxidized flavodiiron protein + NADH
-
 Entamoeba histolytica HM1-IMSS reduced flavodiiron protein + NAD+ + H+
-
 ?
1.18.1.4 oxidized flavodiiron protein + NADPH
-
 Entamoeba histolytica reduced flavodiiron protein + NADP+ + H+
-
 ?
1.18.1.4 oxidized flavodiiron protein + NADPH
-
 Entamoeba histolytica HM1-IMSS reduced flavodiiron protein + NADP+ + H+
-
 ?
1.18.1.4 oxidized Pyrococcus furiosus rubredoxin + NADH
-
 Entamoeba histolytica reduced Pyrococcus furiosus rubredoxin + NAD+ + H+
-
 ?
1.18.1.4 oxidized Pyrococcus furiosus rubredoxin + NADPH
-
 Entamoeba histolytica reduced Pyrococcus furiosus rubredoxin + NADP+ + H+
-
 ?
1.18.1.4 oxidized rubrerythrin + NADH
-
 Entamoeba histolytica reduced rubrerythrin + NAD+ + H+
-
 ?
1.18.1.4 oxidized rubrerythrin + NADPH
-
 Entamoeba histolytica reduced rubrerythrin + NADP+ + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.1.4 monomer 1 * 50000, SDS-PAGE Entamoeba histolytica
1.18.1.4 monomer 1 * 49700, calculated from amino acid sequence Entamoeba histolytica

Synonyms

EC Number Synonyms Comment Organism
1.18.1.4 NAD(P)H-dependent rubredoxin reductase
-
 Entamoeba histolytica
1.18.1.4 NROR
-
 Entamoeba histolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.18.1.4 30
-
-
 Entamoeba histolytica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.4 3 6 oxidized Clostridium pasteurianum rubredoxin with NADH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
1.18.1.4 9.6
-
 oxidized Pyrococcus furiosus rubredoxin with NADPH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
1.18.1.4 13.9
-
 oxidized Clostridium pasteurianum rubredoxin with NADPH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
1.18.1.4 69.4
-
 oxidized Pyrococcus furiosus rubredoxin with NADH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.18.1.4 7
-
-
 Entamoeba histolytica

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.4 FAD
-
 Entamoeba histolytica
1.18.1.4 NADH the catalytic efficiency exhibited by this enzyme for NADH is higher than for NADPH Entamoeba histolytica
1.18.1.4 NADPH the catalytic efficiency exhibited by this enzyme for NADH is higher than for NADPH Entamoeba histolytica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.18.1.4 Entamoeba histolytica calculated from amino acid sequence
-
 8.2

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.18.1.4 3000
-
 oxidized Pyrococcus furiosus rubredoxin with NADPH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
1.18.1.4 5000
-
 oxidized Clostridium pasteurianum rubredoxin with NADPH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
1.18.1.4 8400
-
 oxidized Clostridium pasteurianum rubredoxin with NADH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
1.18.1.4 13000
-
 oxidized Pyrococcus furiosus rubredoxin with NADH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica