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Interaction of photosystem I from Phaeodactylum tricornutum with plastocyanins as compared with its native cytochrome c6 reunion with a lost donor

Bernal-Bayard, P.; Pallara, C.; Carmen Castell, M.; Molina-Heredia, F.; Fernandez-Recio, J.; Hervas, M.; Navarro, J.; Biochim. Biophys. Acta 1847, 1549-1559 (2015)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.97.1.12
additional information
-
additional information
kinetic analysis of cytochrome c6 and different plastocyanins as electron donor cofactors, detailed overview
Phaeodactylum tricornutum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.97.1.12
reduced cytochrome c6 + oxidized ferredoxin + hv
Phaeodactylum tricornutum
-
oxidized cytochrome c6 + reduced ferredoxin
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.97.1.12
Phaeodactylum tricornutum
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.97.1.12
additional information
in the Phaeodactylum tricornutum alga, as inmost diatoms, cytochrome c6 is the only electron donor to photosystem I, and thus they lack plastocyanin as an alternative electron carrier. Analysis of electron transfer to Phaeodactylum photosystem I from several plastocyanins from cyanobacteria, green algae and plants, as compared with its own cytochrome c6, overview. Diatom photosystem I is able to effectively react with eukaryotic acidic plastocyanins, although with less efficiency than with Phaeodactylum cytochrome c6. This efficiency increases in some green alga plastocyanin mutants mimicking the electrostatics of the interaction site on the diatom cytochrome. In addition, the structure of the transient electron transfer complex between cytochrome c6 and photosystem I from Phaeodactylum is analyzed by computational docking and compared to that of green lineage and mixed systems. The Phaeodactylum system shows a lower efficiency than the green systems, both in the formation of the properly arranged [cytochrome c6-photosystem I] complex and in the electron transfer itself. Structural modeling, overview
744392
Phaeodactylum tricornutum
?
-
-
-
-
1.97.1.12
reduced cytochrome c6 + oxidized ferredoxin + hv
-
744392
Phaeodactylum tricornutum
oxidized cytochrome c6 + reduced ferredoxin
-
-
-
?
1.97.1.12
reduced plastocyanin + oxidized ferredoxin + hv
plastocyanins from Nostoc sp. PCC 7119, Monoraphidium braunii, Arabidopsis thaliana, Spinacia oleracea, and wild-type and mutant (E85K, Q88R, E85K/Q88R, E85V, and V93K) plastocyanins from Chlamydomonas reinhardtii, docking simulations and modeling
744392
Phaeodactylum tricornutum
oxidized plastocyanin + reduced ferredoxin
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.97.1.12
7.5
-
assay at
Phaeodactylum tricornutum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.97.1.12
additional information
-
additional information
kinetic analysis of cytochrome c6 and different plastocyanins as electron donor cofactors, detailed overview
Phaeodactylum tricornutum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.97.1.12
reduced cytochrome c6 + oxidized ferredoxin + hv
Phaeodactylum tricornutum
-
oxidized cytochrome c6 + reduced ferredoxin
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.97.1.12
additional information
in the Phaeodactylum tricornutum alga, as inmost diatoms, cytochrome c6 is the only electron donor to photosystem I, and thus they lack plastocyanin as an alternative electron carrier. Analysis of electron transfer to Phaeodactylum photosystem I from several plastocyanins from cyanobacteria, green algae and plants, as compared with its own cytochrome c6, overview. Diatom photosystem I is able to effectively react with eukaryotic acidic plastocyanins, although with less efficiency than with Phaeodactylum cytochrome c6. This efficiency increases in some green alga plastocyanin mutants mimicking the electrostatics of the interaction site on the diatom cytochrome. In addition, the structure of the transient electron transfer complex between cytochrome c6 and photosystem I from Phaeodactylum is analyzed by computational docking and compared to that of green lineage and mixed systems. The Phaeodactylum system shows a lower efficiency than the green systems, both in the formation of the properly arranged [cytochrome c6-photosystem I] complex and in the electron transfer itself. Structural modeling, overview
744392
Phaeodactylum tricornutum
?
-
-
-
-
1.97.1.12
reduced cytochrome c6 + oxidized ferredoxin + hv
-
744392
Phaeodactylum tricornutum
oxidized cytochrome c6 + reduced ferredoxin
-
-
-
?
1.97.1.12
reduced plastocyanin + oxidized ferredoxin + hv
plastocyanins from Nostoc sp. PCC 7119, Monoraphidium braunii, Arabidopsis thaliana, Spinacia oleracea, and wild-type and mutant (E85K, Q88R, E85K/Q88R, E85V, and V93K) plastocyanins from Chlamydomonas reinhardtii, docking simulations and modeling
744392
Phaeodactylum tricornutum
oxidized plastocyanin + reduced ferredoxin
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.97.1.12
7.5
-
assay at
Phaeodactylum tricornutum
General Information
EC Number
General Information
Commentary
Organism
1.97.1.12
additional information
cytochrome c6 in the Phaeodactylum tricornutum alga, as inmost diatoms, is the only electron donor to photosystem I, and thus they lack plastocyanin as an alternative electron carrier
Phaeodactylum tricornutum
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.97.1.12
additional information
cytochrome c6 in the Phaeodactylum tricornutum alga, as inmost diatoms, is the only electron donor to photosystem I, and thus they lack plastocyanin as an alternative electron carrier
Phaeodactylum tricornutum