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Literature summary extracted from

  • Goblirsch, B.; Frias, J.; Wackett, L.; Wilmot, C.
    Crystal structures of Xanthomonas campestris OleA reveal features that promote head-to-head condensation of two long-chain fatty acids (2012), Biochemistry, 51, 4138-4146 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.20
-
Xanthomonas campestris pv. campestris

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.3.20 hanging drop vapor diffusion method, using 16-21% (w/v) PEG 8000, 70-100 mM potassium phosphate dibasic, and 100 mM sodium citrate pH 4.2 (enzyme only) or 13-18% (w/v) PEG 4000, 80-100 mM manganese chloride, and 100 mM MES 6.0 (enzyme in complex with cerulenin) Xanthomonas campestris pv. campestris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.20 cerulenin complete inhibition at 6 mM Xanthomonas campestris pv. campestris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.20 an acyl-CoA + H2O Xanthomonas campestris pv. campestris
-
a (2R)-2-alkyl-3-oxoalkanoate + CoA
-
?
2.3.3.20 an acyl-CoA + H2O Xanthomonas campestris pv. campestris ATCC 33913
-
a (2R)-2-alkyl-3-oxoalkanoate + CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.20 Xanthomonas campestris pv. campestris Q8PDX2
-
-
2.3.3.20 Xanthomonas campestris pv. campestris ATCC 33913 Q8PDX2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.20
-
Xanthomonas campestris pv. campestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.20 an acyl-CoA + H2O
-
Xanthomonas campestris pv. campestris a (2R)-2-alkyl-3-oxoalkanoate + CoA
-
?
2.3.3.20 an acyl-CoA + H2O
-
Xanthomonas campestris pv. campestris ATCC 33913 a (2R)-2-alkyl-3-oxoalkanoate + CoA
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.20 oleA
-
Xanthomonas campestris pv. campestris