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Literature summary extracted from

  • Hasegawa, S.; Imai, M.; Yamasaki, M.; Takahashi, N.; Fukui, T.
    Transcriptional regulation of acetoacetyl-CoA synthetase by Sp1 in neuroblastoma cells (2018), Biochem. Biophys. Res. Commun., 495, 652-658 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.16 gene AACS, analysis of the transcriptional mechanism of AACS in Neuro-2a neuroblastoma cells. The minimal core promoter of the mouse AACS gene is located in a region with 110 bps upstream from the transcription start site. Mutagenesis studies show that the Sp1 binding site is crucial for AACS promoter activity. Sp1 binds to the Sp1 binding site on the promoter region of AACS. Overexpression of Sp1 increases AACS mRNA levels. Real-time PCR enzyme expression analysis. Functional analysis of the 5'-flanking region of AACS in Neuro-2a cells Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.16 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.16 ATP + acetoacetate + CoA Homo sapiens
-
 AMP + diphosphate + acetoacetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.16 Homo sapiens Q86V21
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.16 Neuro-2a cell
-
 Homo sapiens
-
6.2.1.16 neuroblastoma cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.16 ATP + acetoacetate + CoA
-
 Homo sapiens AMP + diphosphate + acetoacetyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.16 AACS
-
 Homo sapiens
6.2.1.16 Acetoacetyl-CoA synthetase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.16 ATP
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
6.2.1.16 Homo sapiens specificity protein 1 (Sp1) regulates gene expression of AACS in Neuro-2a cells, investigation of promoter activity of AACS, overview additional information
6.2.1.16 Homo sapiens specificity protein 1 (Sp1) binds to the Sp1 binding site on the promoter region of AACS. Overexpression of Sp1 increases AACS mRNA levels up

General Information

EC Number General Information Comment Organism
6.2.1.16 malfunction knockdown of SREBP-2, which orchestrates cholesterol synthesis, results in the downregulation of AACS mRNA levels. Knockdown of AACS results in a decrease in histone deacetylase 9, associated with gene silencing Homo sapiens
6.2.1.16 physiological function acetoacetyl-CoA synthetase (AACS) is the enzyme responsible for cholesterol and fatty acid synthesis in the cytosol. AACS has an important role in normal neuronal development. Specificity protein 1 (Sp1) regulates gene expression of AACS in Neuro-2a cells and ketone body utilization affects the balance of histone acetylation Homo sapiens