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Literature summary extracted from

  • Shen, Y.; Pan, X.; Zhao, H.
    The histone demethylase PHF8 is an oncogenic protein in human non-small cell lung cancer (2014), Biochem. Biophys. Res. Commun., 451, 119-125 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.65 gene PHF8, quantitative real-time PCR enzyme expression analysis Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.65 additional information enzyme knockdown by expression of specific sh-RNAs targeting PHF8 in HEK 293 T cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.65 nucleus
-
 Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.65 histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2 Homo sapiens
-
 histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2
-
 ?
1.14.11.65 histone H3 N6-methyl-L-lysine9 + 2-oxoglutarate + O2 Homo sapiens
-
 histone H3 L-lysine9 + succinate + formaldehyde + CO2
-
 ?
1.14.11.65 histone H4 N6-methyl-L-lysine20 + 2-oxoglutarate + O2 Homo sapiens
-
 histone H4 L-lysine20 + succinate + formaldehyde + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.65 Homo sapiens Q9UPP1
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.65 A-549 cell
-
 Homo sapiens
-
1.14.11.65 NCI-H292 cell
-
 Homo sapiens
-
1.14.11.65 non-small cell lung cancer cell
-
 Homo sapiens
-
1.14.11.65 RERF-LC-AI cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.65 histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2
-
 Homo sapiens histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2
-
 ?
1.14.11.65 histone H3 N6-methyl-L-lysine9 + 2-oxoglutarate + O2
-
 Homo sapiens histone H3 L-lysine9 + succinate + formaldehyde + CO2
-
 ?
1.14.11.65 histone H4 N6-methyl-L-lysine20 + 2-oxoglutarate + O2
-
 Homo sapiens histone H4 L-lysine20 + succinate + formaldehyde + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.65 histone demethylase
-
 Homo sapiens
1.14.11.65 PHF8
-
 Homo sapiens
1.14.11.65 plant homeodomain finger protein 8
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
1.14.11.65 malfunction PHF8 knockdown induces DNA damage and apoptosis in lung cancer cells. miR-21 knockdown blocks the effects of PHF8 on proliferation and apoptosis of lung cancer cells. Cell proliferation and colonyforming activity are markedly reduced when PHF8 is knocked down in A-549, LC-AI, and NCI-H292 cells Homo sapiens
1.14.11.65 physiological function PHF8 is a JmjC domain-containing protein and erases repressive histone marks including H4K20me1 and H3K9me1/2. It binds to H3K4me3, an active histone mark usually located at transcription start sites (TSSs), through its plant homeo-domain, and is thus recruited and enriched in gene promoters. PHF8 is involved in the development of several types of cancer, including leukemia, prostate cancer, and esophageal squamous cell carcinoma. PHF8 promotes miR-21 expression in human lung cancer, and PHF8 promotes lung cancer cell growth and survival by regulating miR-21. PHF8 is an oncogenic protein in human non-small cell lung cancer (NSCLC) and is upregulated in human NSCLC tissues. PHF8 regulates lung cancer cell growth and transformation. High PHF8 expression predicts poor survival Homo sapiens