Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sun, J.; Kweon, O.; Jin, J.; He, G.; Li, X.; Cerniglia, C.; Chen, H.
    Mutation network-based understanding of pleiotropic and epistatic mutational behavior of Enterococcus faecalis FMN-dependent azoreductase (2017), Biochem. Biophys. Rep., 12, 240-244 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.17 expression in Escherichia coli Enterococcus faecalis

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.17 N121Q 115% of wild-type activity Enterococcus faecalis
1.7.1.17 R21G/N121A 136% of wild-type activity Enterococcus faecalis
1.7.1.17 R21G/N121Q 171% of wild-type activity Enterococcus faecalis
1.7.1.17 R21K 80% of wild-type activity Enterococcus faecalis
1.7.1.17 R21K/N121A 41% of wild-type activity Enterococcus faecalis
1.7.1.17 R21K/N121Q 40% of wild-type activity Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.17 0.011
-
 4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant R21G/N121A, pH 7.1, temperature not specified in the publication Enterococcus faecalis
1.7.1.17 0.016
-
 4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant R21G/N121Q, pH 7.1, temperature not specified in the publication Enterococcus faecalis
1.7.1.17 0.02
-
 4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant R21K, pH 7.1, temperature not specified in the publication Enterococcus faecalis
1.7.1.17 0.024
-
 4'-(dimethylamino)-azobenzene-2-carboxylic acid wild-type, pH 7.1, temperature not specified in the publication Enterococcus faecalis
1.7.1.17 0.029
-
 4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant N121Q, pH 7.1, temperature not specified in the publication Enterococcus faecalis
1.7.1.17 0.031
-
 4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant R21K/N121A, pH 7.1, temperature not specified in the publication Enterococcus faecalis
1.7.1.17 0.034
-
 4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant R21K/N121Q, pH 7.1, temperature not specified in the publication Enterococcus faecalis
1.7.1.17 0.08
-
 NADH mutant R21G/N121A, pH 7.1, temperature not specified in the publication Enterococcus faecalis
1.7.1.17 0.115
-
 NADH mutant R21K/N121Q, pH 7.1, temperature not specified in the publication Enterococcus faecalis
1.7.1.17 0.122
-
 NADH mutant R21K, pH 7.1, temperature not specified in the publication Enterococcus faecalis
1.7.1.17 0.143
-
 NADH wild-type, pH 7.1, temperature not specified in the publication Enterococcus faecalis
1.7.1.17 0.19
-
 NADH mutant R21G/N121Q, pH 7.1, temperature not specified in the publication Enterococcus faecalis
1.7.1.17 0.267
-
 NADH mutant R21K/N121Q, pH 7.1, temperature not specified in the publication Enterococcus faecalis
1.7.1.17 0.371
-
 NADH mutant N121Q, pH 7.1, temperature not specified in the publication Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.17 Enterococcus faecalis Q831B2
-
-
1.7.1.17 Enterococcus faecalis ATCC 700802 Q831B2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Enterococcus faecalis 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+
-
 ?
1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Enterococcus faecalis ATCC 700802 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.17 ? x * 23000, SDS-PAGE Enterococcus faecalis

Synonyms

EC Number Synonyms Comment Organism
1.7.1.17 AzoR
-
 Enterococcus faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.17 NADH
-
 Enterococcus faecalis