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Literature summary extracted from

  • Terrasan, C.; Aragon, C.; Masui, D.; Pessela, B.; Fernandez-Lorente, G.; Carmona, E.; Guisan, J.
    beta-Xylosidase from Selenomonas ruminantium Immobilization, stabilization, and application for xylooligosaccharide hydrolysis (2016), Biocatal. Biotransform., 34, 161-171 .
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.37 coating the enzyme with aldehyde-dextran or polyethylenimine, fully stabilizes the quaternary structure of the enzyme rendering much more stabilization to the biocatalyst. The enzyme coated with polyethylenimine of molecular weight 1300 is the most stable one exhibiting an half-life of more than 10 days at pH 5.0 and 50°C, being 240fold more stable than the free enzyme Selenomonas ruminantium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 D-glucose
-
 Selenomonas ruminantium
3.2.1.37 D-xylose
-
 Selenomonas ruminantium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.45
-
 4-nitrophenyl beta-D-xylopyranoside pH and temperature not specified in the publication Selenomonas ruminantium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Selenomonas ruminantium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Selenomonas ruminantium 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Selenomonas ruminantium 4-nitrophenol + beta-D-xylopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 homotetramer
-
 Selenomonas ruminantium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 beta-xylosidase
-
 Selenomonas ruminantium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 55
-
 enzyme coated with polyethylenimine shows optimum activity at 55°C Selenomonas ruminantium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 0.82
-
 4-nitrophenyl beta-D-xylopyranoside pH and temperature not specified in the publication Selenomonas ruminantium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.37 4 6 enzyme coated with polyethylenimine shows optimum activity in the pH range 4.0–6.0 Selenomonas ruminantium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.37 1.82
-
 4-nitrophenyl beta-D-xylopyranoside pH and temperature not specified in the publication Selenomonas ruminantium