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Literature summary extracted from

  • Evans, A.; Ribble, W.; Schexnaydre, E.; Waldrop, G.L.
    Acetyl-CoA carboxylase from Escherichia coli exhibits a pronounced hysteresis when inhibited by palmitoyl-acyl carrier protein (2017), Arch. Biochem. Biophys., 636, 100-109 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.15 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.15 malonyl-CoA product inhibition Escherichia coli
2.1.3.15 palmitoyl-acyl carrier protein allosteric, time-dependent, competitive inhibition Escherichia coli
2.1.3.15 pantothenic acid potent inhibitor Escherichia coli
6.4.1.2 palmitoyl-acyl carrier protein 5 min, 55% inhibition, decrease of inhibition thereafter. Enzyme exhibits a significant hysteresis in presence of palmitoyl-acyl carrier protein. Allosteric inhibitor, the pantothenic acid moiety of palmitoyl-acyl carrier protein is responsible for the inhibition. Competitive with respect to acetyl-CoA Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.15 [biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA Escherichia coli
-
 [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.15 Escherichia coli
-
-
-
6.4.1.2 Escherichia coli P0ABD5 and P0A9Q5 P0ABD5 i.e. subunit alpha, P0A9Q5 i.e. subunit beta
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.15
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.15 [biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA
-
 Escherichia coli [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.1.3.15 ACC
-
 Escherichia coli
2.1.3.15 acetyl-CoA carboxylase
-
 Escherichia coli
6.4.1.2 AccA subunit alpha Escherichia coli
6.4.1.2 ACCB subunit beta Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.3.15 ATP
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.15 0.0439
-
 pantothenic acid at pH 7.8 and 25°C Escherichia coli
2.1.3.15 0.088
-
 palmitoyl-acyl carrier protein at pH 7.8 and 25°C Escherichia coli
6.4.1.2 0.088
-
 palmitoyl-acyl carrier protein pH 7.8, temperature not specified in the publication Escherichia coli