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Literature summary extracted from

  • Rimal, H.; Lee, S.; Lee, J.; Oh, T.
    Understanding of real alternative redox partner of Streptomyces peucetius DoxA Prediction and validation using in silico and in vitro analyses (2015), Arch. Biochem. Biophys., 585, 64-74 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.181 recombinant overexpression of His-tagged enzyme DoxA along with its His-tagged redox partners from Streptomyces peucetius FDX1, FDR2, and FDX3, and the putidaredoxin and putidaredoxin reductase from Pseudomonas putida, that are essential equivalents of the class I type of bacterial electron-transport system, in Escherichia coli strain BL21(DE3) harboring the GroES/EL-expressing plasmid pGro7 Streptomyces peucetius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.181 daunorubicin + NADPH + H+ + O2 Streptomyces peucetius
-
 doxorubicin + NADP+ + H2O
-
 ?
1.14.13.181 daunorubicin + NADPH + H+ + O2 Streptomyces peucetius ATCC 27952
-
 doxorubicin + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.181 Streptomyces peucetius
-
-
-
1.14.13.181 Streptomyces peucetius ATCC 27952
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.181 recombinant His-tagged enzyme DoxA from Escherichia coli, copurification with His-tagged FDX1, FDR2, FDX3, and putidaredoxin and putidaredoxin reductase from Pseudomonas putida from Escherichia coli by cobalt affinity chromatography and ultrafiltration Streptomyces peucetius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.181 daunorubicin + NADPH + H+ + O2
-
 Streptomyces peucetius doxorubicin + NADP+ + H2O
-
 ?
1.14.13.181 daunorubicin + NADPH + H+ + O2
-
 Streptomyces peucetius ATCC 27952 doxorubicin + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.181 DoxA
-
 Streptomyces peucetius

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.181 cytochrome P450
-
 Streptomyces peucetius
1.14.13.181 NADPH
-
 Streptomyces peucetius

General Information

EC Number General Information Comment Organism
1.14.13.181 additional information protein-protein interaction and docking analysis of recombinant enzymes, structure homology modeling, DoxA active site structure, overview. Enzyme DoxA interacts with ist redox partners FDX1, FDR2, and FDX3 Streptomyces peucetius
1.14.13.181 physiological function the cytochrome P450 monoxygenase DoxA is responsible for the hydroxylation of daunorubicin into doxorubicin Streptomyces peucetius