Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Robinson, R.M.; Rodriguez, P.J.; Sobrado, P.
    Mechanistic studies on the flavin-dependent N6-lysine monooxygenase MbsG reveal an unusual control for catalysis (2014), Arch. Biochem. Biophys., 550-551, 58-66 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.59 additional information
-
 additional information analysis of steady-state and rapid reaction conditions using primary and solvent kinetic isotope effects, stopped-flow and steady-state kinetics, overview Mycolicibacterium smegmatis
1.14.13.59 2.4
-
 L-lysine pH 7.5, 25°C, with NADH Mycolicibacterium smegmatis
1.14.13.59 12
-
 L-lysine pH 7.5, 25°C, with NADPH Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.59 L-lysine + NADH + H+ + O2 Mycolicibacterium smegmatis
-
 N6-hydroxy-L-lysine + NAD+ + H2O
-
 ?
1.14.13.59 L-lysine + NADH + H+ + O2 Mycolicibacterium smegmatis G
-
 N6-hydroxy-L-lysine + NAD+ + H2O
-
 ?
1.14.13.59 L-lysine + NADPH + H+ + O2 Mycolicibacterium smegmatis
-
 N6-hydroxy-L-lysine + NADP+ + H2O
-
 ?
1.14.13.59 L-lysine + NADPH + H+ + O2 Mycolicibacterium smegmatis G
-
 N6-hydroxy-L-lysine + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.59 Mycolicibacterium smegmatis
-
-
-
1.14.13.59 Mycolicibacterium smegmatis G
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.59 L-lysine + NADPH + H+ + O2 = N6-hydroxy-L-lysine + NADP+ + H2O unique mechanism where a rate-limiting and pH-sensitive conformational change occurs in the reductive half-reaction, which affects the efficiency of lysine hydroxylation Mycolicibacterium smegmatis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.59 L-lysine + NADH + H+ + O2
-
 Mycolicibacterium smegmatis N6-hydroxy-L-lysine + NAD+ + H2O
-
 ?
1.14.13.59 L-lysine + NADH + H+ + O2 the hydride transfer is rate-limiting Mycolicibacterium smegmatis N6-hydroxy-L-lysine + NAD+ + H2O
-
 ?
1.14.13.59 L-lysine + NADH + H+ + O2
-
 Mycolicibacterium smegmatis G N6-hydroxy-L-lysine + NAD+ + H2O
-
 ?
1.14.13.59 L-lysine + NADH + H+ + O2 the hydride transfer is rate-limiting Mycolicibacterium smegmatis G N6-hydroxy-L-lysine + NAD+ + H2O
-
 ?
1.14.13.59 L-lysine + NADPH + H+ + O2
-
 Mycolicibacterium smegmatis N6-hydroxy-L-lysine + NADP+ + H2O
-
 ?
1.14.13.59 L-lysine + NADPH + H+ + O2 the hydride transfer is rate-limiting Mycolicibacterium smegmatis N6-hydroxy-L-lysine + NADP+ + H2O
-
 ?
1.14.13.59 L-lysine + NADPH + H+ + O2
-
 Mycolicibacterium smegmatis G N6-hydroxy-L-lysine + NADP+ + H2O
-
 ?
1.14.13.59 L-lysine + NADPH + H+ + O2 the hydride transfer is rate-limiting Mycolicibacterium smegmatis G N6-hydroxy-L-lysine + NADP+ + H2O
-
 ?
1.14.13.59 additional information MbsG is non-specific for reduced pyridine dinucleotide, as it can utilize both NADH and NADPH with similar catalytic efficiency. But MbsG is specific for L-lysine and shows no activity with L-ornithine, 6-amino-1-hexanol, L-arginine, putrescine, and cadaverine Mycolicibacterium smegmatis ?
-
 ?
1.14.13.59 additional information MbsG is non-specific for reduced pyridine dinucleotide, as it can utilize both NADH and NADPH with similar catalytic efficiency. But MbsG is specific for L-lysine and shows no activity with L-ornithine, 6-amino-1-hexanol, L-arginine, putrescine, and cadaverine Mycolicibacterium smegmatis G ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.59 flavin-dependent N6-lysine monooxygenase
-
 Mycolicibacterium smegmatis
1.14.13.59 MbsG
-
 Mycolicibacterium smegmatis
1.14.13.59 N-hydroxylating monooxygenase
-
 Mycolicibacterium smegmatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.59 25
-
 assay at Mycolicibacterium smegmatis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.59 0.43
-
 L-lysine pH 7.5, 25°C, with NADH Mycolicibacterium smegmatis
1.14.13.59 0.55
-
 L-lysine pH 7.5, 25°C, with NADPH Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.59 7.5
-
 assay at Mycolicibacterium smegmatis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.59 5.4 9 stable higher activity within this range Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.59 FAD dependent on Mycolicibacterium smegmatis
1.14.13.59 additional information activity measurements with isotopes of NAD(P)H, kinetic isotope effects, overview Mycolicibacterium smegmatis
1.14.13.59 NADH
-
 Mycolicibacterium smegmatis
1.14.13.59 NADPH
-
 Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
1.14.13.59 physiological function Mycobacterium smegmatis G (MbsG) is a flavin-dependent L-lysine monooxygenase involved in the biosynthesis of the siderophore mycobactin, overview Mycolicibacterium smegmatis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.13.59 0.046
-
 L-lysine pH 7.5, 25°C, with NADPH Mycolicibacterium smegmatis
1.14.13.59 0.179
-
 L-lysine pH 7.5, 25°C, with NADH Mycolicibacterium smegmatis