Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Oliver, J.C.; Gudihal, R.; Burgner, J.W.; Pedley, A.M.; Zwierko, A.T.; Davisson, V.J.; Linger, R.S.
    Conformational changes involving ammonia tunnel formation and allosteric control in GMP synthetase (2014), Arch. Biochem. Biophys., 545, 22-32 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.5.2 structural model of GMPS in a closed, active state. The salt bridge between residues H186 and E383 functions as a connection between the two active sites Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.2 E383A 8fold decrease in glutaminase activity Escherichia coli
6.3.5.2 H186A 30-50 fold decrease in Km value for glutamine Escherichia coli
6.3.5.2 H186A/E383A mutant exhibits poor solubility and basal glutaminase activity Escherichia coli
6.3.5.2 H186E/E383H interchange of the amino acids of the salt bridge, significant decrease in catalytic activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.2 0.007
-
 XMP mutant H186A/E383A, overall reaction, pH 8.5, 37°C Escherichia coli
6.3.5.2 0.008
-
 XMP mutant H186E/E383H, overall reaction, pH 8.5, 37°C Escherichia coli
6.3.5.2 0.022
-
 XMP mutant E383A, overall reaction, pH 8.5, 37°C Escherichia coli
6.3.5.2 0.043
-
 XMP wild-type, release of NH3, pH 8.5, 37°C Escherichia coli
6.3.5.2 0.045
-
 XMP mutant H186A, overall reaction, pH 8.5, 37°C Escherichia coli
6.3.5.2 0.05
-
 XMP mutant H186A, release of NH3, pH 8.5, 37°C Escherichia coli
6.3.5.2 0.05
-
 XMP mutant H186E/E383H, release of NH3, pH 8.5, 37°C Escherichia coli
6.3.5.2 0.053
-
 XMP wild-type, overall reaction, pH 8.5, 37°C Escherichia coli
6.3.5.2 0.056
-
 XMP mutant E383A, release of NH3, pH 8.5, 37°C Escherichia coli
6.3.5.2 0.074
-
 XMP mutant H186A/E383A, release of NH3, pH 8.5, 37°C Escherichia coli
6.3.5.2 1.6
-
 L-glutamine wild-type, pH 8.5, 37°C Escherichia coli
6.3.5.2 3.2
-
 L-glutamine mutant E383A, pH 8.5, 37°C Escherichia coli
6.3.5.2 67
-
 L-glutamine mutant H186E/E383H, pH 8.5, 37°C Escherichia coli
6.3.5.2 80
-
 L-glutamine mutant H186A, pH 8.5, 37°C Escherichia coli
6.3.5.2 99
-
 L-glutamine mutant H186A/E383A, pH 8.5, 37°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.2 Escherichia coli P04079
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.2 ATP + XMP + L-glutamine + H2O
-
 Escherichia coli AMP + diphosphate + GMP + L-glutamate
-
 ?
6.3.5.2 ATP + XMP + NH3
-
 Escherichia coli AMP + diphosphate + GMP
-
 ?
6.3.5.2 L-glutamine + H2O
-
 Escherichia coli L-glutamate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.5.2 GMPS
-
 Escherichia coli
6.3.5.2 GuaA
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.5.2 0.081
-
 XMP mutant H186E/E383H, overall reaction, pH 8.5, 37°C Escherichia coli
6.3.5.2 0.085
-
 XMP mutant H186A/E383A, overall reaction, pH 8.5, 37°C Escherichia coli
6.3.5.2 3.2
-
 XMP mutant E383A, overall reaction, pH 8.5, 37°C Escherichia coli
6.3.5.2 3.2
-
 L-glutamine mutant E383A, pH 8.5, 37°C Escherichia coli
6.3.5.2 4.7
-
 L-glutamine mutant H186E/E383H, pH 8.5, 37°C Escherichia coli
6.3.5.2 4.8
-
 L-glutamine mutant H186A/E383A, pH 8.5, 37°C Escherichia coli
6.3.5.2 6.1
-
 XMP mutant H186E/E383H, release of NH3, pH 8.5, 37°C Escherichia coli
6.3.5.2 7
-
 XMP mutant E383A, release of NH3, pH 8.5, 37°C Escherichia coli
6.3.5.2 9
-
 XMP mutant H186A/E383A, release of NH3, pH 8.5, 37°C Escherichia coli
6.3.5.2 13.3
-
 XMP wild-type, release of NH3, pH 8.5, 37°C Escherichia coli
6.3.5.2 13.7
-
 L-glutamine wild-type, pH 8.5, 37°C Escherichia coli
6.3.5.2 14
-
 XMP mutant H186A, overall reaction, pH 8.5, 37°C Escherichia coli
6.3.5.2 15.6
-
 XMP mutant H186A, release of NH3, pH 8.5, 37°C Escherichia coli
6.3.5.2 16
-
 L-glutamine mutant H186A, pH 8.5, 37°C Escherichia coli
6.3.5.2 23
-
 XMP wild-type, overall reaction, pH 8.5, 37°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.3.5.2 0.049
-
 L-glutamine mutant H186A/E383A, pH 8.5, 37°C Escherichia coli
6.3.5.2 0.07
-
 L-glutamine mutant H186E/E383H, pH 8.5, 37°C Escherichia coli
6.3.5.2 0.21
-
 L-glutamine mutant H186A, pH 8.5, 37°C Escherichia coli
6.3.5.2 1
-
 L-glutamine mutant E383A, pH 8.5, 37°C Escherichia coli
6.3.5.2 8
-
 L-glutamine wild-type, pH 8.5, 37°C Escherichia coli
6.3.5.2 100
-
 XMP mutant H186E/E383H, overall reaction, pH 8.5, 37°C Escherichia coli
6.3.5.2 110
-
 XMP mutant H186A/E383A, overall reaction, pH 8.5, 37°C Escherichia coli
6.3.5.2 120
-
 XMP mutant E383A, release of NH3, pH 8.5, 37°C Escherichia coli
6.3.5.2 120
-
 XMP mutant H186A/E383A, release of NH3, pH 8.5, 37°C Escherichia coli
6.3.5.2 120
-
 XMP mutant H186E/E383H, release of NH3, pH 8.5, 37°C Escherichia coli
6.3.5.2 140
-
 XMP mutant E383A, overall reaction, pH 8.5, 37°C Escherichia coli
6.3.5.2 310
-
 XMP mutant H186A, overall reaction, pH 8.5, 37°C Escherichia coli
6.3.5.2 310
-
 XMP mutant H186A, release of NH3, pH 8.5, 37°C Escherichia coli
6.3.5.2 310
-
 XMP wild-type, release of NH3, pH 8.5, 37°C Escherichia coli
6.3.5.2 430
-
 XMP wild-type, overall reaction, pH 8.5, 37°C Escherichia coli