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Literature summary extracted from
- Improvement of thermostability and activity of Trichoderma reesei endo-xylanase Xyn III on insoluble substrates (2016), Appl. Microbiol. Biotechnol., 100, 8043-8051 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.8 | expression in Escherichia coli | Trichoderma reesei |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.8 | additional information | the addition of a xylan-binding domain to the C-terminus of Xyn III improves its hydrolytic activity on insoluble xylan | Trichoderma reesei |
| 3.2.1.8 | Q286A/N340Y | substantial improvement of thermostability | Trichoderma reesei |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.8 | Trichoderma reesei | LC132960 | codon-optimized gene | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 19.6 | - |
wild-type, substrate insoluble oat spelt xylan, pH 6.0, 50°C | Trichoderma reesei |
| 3.2.1.8 | 49.3 | - |
mutant Q286A/N340Y, substrate insoluble oat spelt xylan, pH 6.0, 50°C | Trichoderma reesei |
| 3.2.1.8 | 142 | - |
mutant Q286A/N340Y, substrate soluble oat spelt xylan, pH 6.0, 50°C | Trichoderma reesei |
| 3.2.1.8 | 143 | - |
wild-type, substrate soluble oat spelt xylan, pH 6.0, 50°C | Trichoderma reesei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.8 | birchwood xylan + H2O | - |
Trichoderma reesei | ? | - |
? |  |
| 3.2.1.8 | insoluble oat spelt xylan + H2O | - |
Trichoderma reesei | ? | - |
? | |
| 3.2.1.8 | soluble oat spelt xylan + H2O | - |
Trichoderma reesei | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.8 | Xyn III | - |
Trichoderma reesei |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 55 | - |
wild-type | Trichoderma reesei |
| 3.2.1.8 | 60 | - |
mutant Q286A/N340Y | Trichoderma reesei |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 55 | - |
30 min, almost complete loss of activity for wild-type, 9% residual activity for mutant Q286A/N340Y | Trichoderma reesei |
| 3.2.1.8 | 60 | - |
30 min, 51% residual activity, mutant Q286A/N340Y | Trichoderma reesei |
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Release 2023.1 (January 2023)
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