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Literature summary extracted from

  • Yang, J.; Li, W.; Wang, D.; Wu, H.; Li, Z.; Ye, Q.
    Characterization of bifunctional L-glutathione synthetases from Actinobacillus pleuropneumoniae and Actinobacillus succinogenes for efficient glutathione biosynthesis (2016), Appl. Microbiol. Biotechnol., 100, 6279-6289 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.2 gene gshF, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Actinobacillus pleuropneumoniae
6.3.2.2 gene gshF, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Actinobacillus succinogenes
6.3.2.3 gene gshF, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Actinobacillus pleuropneumoniae
6.3.2.3 gene gshF, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Actinobacillus succinogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.2 GSH feedback inhibition, the gamma-GCS activity is inhibited by a high concentration of GSH Actinobacillus pleuropneumoniae
6.3.2.2 GSH feedback inhibition, the gamma-GCS activity is inhibited by a high concentration of GSH Actinobacillus succinogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.2 additional information
-
 additional information kinetics Actinobacillus pleuropneumoniae
6.3.2.2 additional information
-
 additional information kinetics Actinobacillus succinogenes
6.3.2.2 0.05
-
 L-cysteine pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus succinogenes
6.3.2.2 0.199
-
 ATP pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus pleuropneumoniae
6.3.2.2 0.385
-
 ATP pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus succinogenes
6.3.2.2 11
-
 L-glutamate pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus succinogenes
6.3.2.2 12
-
 L-glutamate pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus pleuropneumoniae
6.3.2.3 additional information
-
 additional information kinetics Actinobacillus pleuropneumoniae
6.3.2.3 additional information
-
 additional information kinetics Actinobacillus succinogenes
6.3.2.3 0.299
-
 ATP pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus pleuropneumoniae
6.3.2.3 0.356
-
 ATP pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus succinogenes
6.3.2.3 1.13
-
 gamma-L-glutamyl-L-cysteine pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus pleuropneumoniae
6.3.2.3 1.3
-
 glycine pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus pleuropneumoniae
6.3.2.3 1.56
-
 gamma-L-glutamyl-L-cysteine pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus succinogenes
6.3.2.3 2.1
-
 glycine pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus succinogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.2 Mg2+ required Actinobacillus pleuropneumoniae
6.3.2.2 Mg2+ required Actinobacillus succinogenes
6.3.2.3 Mg2+ required Actinobacillus pleuropneumoniae
6.3.2.3 Mg2+ required Actinobacillus succinogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.2 ATP + L-glutamate + L-cysteine Actinobacillus pleuropneumoniae
-
 ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine Actinobacillus succinogenes
-
 ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine Actinobacillus succinogenes ATCC 55618
-
 ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine Actinobacillus pleuropneumoniae ATCC 55618
-
 ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.3 ATP + gamma-L-glutamyl-L-cysteine + glycine Actinobacillus pleuropneumoniae
-
 ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-L-glutamyl-L-cysteine + glycine Actinobacillus succinogenes
-
 ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-L-glutamyl-L-cysteine + glycine Actinobacillus succinogenes ATCC 55618
-
 ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-L-glutamyl-L-cysteine + glycine Actinobacillus pleuropneumoniae ATCC 55618
-
 ADP + phosphate + glutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Actinobacillus pleuropneumoniae D4N892
-
-
6.3.2.2 Actinobacillus succinogenes A6VQP8
-
-
6.3.2.2 Actinobacillus succinogenes ATCC 55618 A6VQP8
-
-
6.3.2.3 Actinobacillus pleuropneumoniae D4N892
-
-
6.3.2.3 Actinobacillus pleuropneumoniae ATCC 55618 D4N892
-
-
6.3.2.3 Actinobacillus succinogenes A6VQP8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.2 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Actinobacillus pleuropneumoniae
6.3.2.2 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Actinobacillus succinogenes
6.3.2.3 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Actinobacillus pleuropneumoniae
6.3.2.3 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Actinobacillus succinogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.2 additional information
-
 GshFAp has a gamma-GCS catalytic activity with Vmax of 820 U/mg Actinobacillus pleuropneumoniae
6.3.2.2 additional information
-
 GshFAs has a high gamma-GCS catalytic activity with Vmax of 1560 U/mg Actinobacillus succinogenes
6.3.2.3 additional information
-
 GshFAp has a glutathione synthetase catalytic activity with Vmax of 775 U/mg Actinobacillus pleuropneumoniae
6.3.2.3 additional information
-
 GshFAp has a high gamma-GCS catalytic activity with Vmax of 1393 U/mg Actinobacillus succinogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-glutamate + L-cysteine
-
 Actinobacillus pleuropneumoniae ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine
-
 Actinobacillus succinogenes ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine
-
 Actinobacillus succinogenes ATCC 55618 ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.2 ATP + L-glutamate + L-cysteine
-
 Actinobacillus pleuropneumoniae ATCC 55618 ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.3 ATP + gamma-L-glutamyl-L-cysteine + glycine
-
 Actinobacillus pleuropneumoniae ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-L-glutamyl-L-cysteine + glycine
-
 Actinobacillus succinogenes ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-L-glutamyl-L-cysteine + glycine
-
 Actinobacillus succinogenes ATCC 55618 ADP + phosphate + glutathione
-
 ?
6.3.2.3 ATP + gamma-L-glutamyl-L-cysteine + glycine
-
 Actinobacillus pleuropneumoniae ATCC 55618 ADP + phosphate + glutathione
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.2 Asuc_1947
-
 Actinobacillus pleuropneumoniae
6.3.2.2 Asuc_1947
-
 Actinobacillus succinogenes
6.3.2.2 bifunctional L-glutathione synthetase
-
 Actinobacillus pleuropneumoniae
6.3.2.2 bifunctional L-glutathione synthetase
-
 Actinobacillus succinogenes
6.3.2.2 gamma-GCS
-
 Actinobacillus pleuropneumoniae
6.3.2.2 glutathione biosynthesis bifunctional protein GshAB UniProt Actinobacillus pleuropneumoniae
6.3.2.2 glutathione biosynthesis bifunctional protein GshAB UniProt Actinobacillus succinogenes
6.3.2.2 gshAB gene name, UniProt Actinobacillus pleuropneumoniae
6.3.2.2 gshAB gene name, UniProt Actinobacillus succinogenes
6.3.2.2 GshF
-
 Actinobacillus pleuropneumoniae
6.3.2.2 GshF
-
 Actinobacillus succinogenes
6.3.2.2 GshFAp
-
 Actinobacillus pleuropneumoniae
6.3.2.2 GshFAs
-
 Actinobacillus succinogenes
6.3.2.3 Asuc_1947
-
 Actinobacillus pleuropneumoniae
6.3.2.3 Asuc_1947
-
 Actinobacillus succinogenes
6.3.2.3 bifunctional L-glutathione synthetase
-
 Actinobacillus pleuropneumoniae
6.3.2.3 bifunctional L-glutathione synthetase
-
 Actinobacillus succinogenes
6.3.2.3 gamma-GCS
-
 Actinobacillus pleuropneumoniae
6.3.2.3 glutathione biosynthesis bifunctional protein GshAB UniProt Actinobacillus pleuropneumoniae
6.3.2.3 glutathione biosynthesis bifunctional protein GshAB UniProt Actinobacillus succinogenes
6.3.2.3 gshAB gene name, UniProt Actinobacillus pleuropneumoniae
6.3.2.3 gshAB gene name, UniProt Actinobacillus succinogenes
6.3.2.3 GshF
-
 Actinobacillus pleuropneumoniae
6.3.2.3 GshF
-
 Actinobacillus succinogenes
6.3.2.3 GshFAp
-
 Actinobacillus pleuropneumoniae
6.3.2.3 GshFAs
-
 Actinobacillus succinogenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.2 40
-
-
 Actinobacillus pleuropneumoniae
6.3.2.2 40
-
-
 Actinobacillus succinogenes
6.3.2.3 40
-
-
 Actinobacillus pleuropneumoniae
6.3.2.3 40
-
-
 Actinobacillus succinogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.2 9
-
-
 Actinobacillus pleuropneumoniae
6.3.2.2 9
-
-
 Actinobacillus succinogenes
6.3.2.3 9
-
-
 Actinobacillus pleuropneumoniae
6.3.2.3 9
-
-
 Actinobacillus succinogenes

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.2 ATP
-
 Actinobacillus pleuropneumoniae
6.3.2.2 ATP
-
 Actinobacillus succinogenes
6.3.2.3 ATP
-
 Actinobacillus pleuropneumoniae
6.3.2.3 ATP
-
 Actinobacillus succinogenes

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.2.2 0.033
-
 GSH pH 8.5, 40°C Actinobacillus pleuropneumoniae
6.3.2.2 0.043
-
 GSH pH 8.5, 40°C, recombinant His-tagged enzyme Actinobacillus succinogenes