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Literature summary extracted from

  • Zhou, J.; Li, Z.; Wu, J.; Li, L.; Li, D.; Ye, X.; Luo, X.; Huang, Y.; Cui, Z.; Cao, H.
    Functional Analysis of a novel beta-(1,3)-glucanase from Corallococcus sp. strain EGB containing a fascin-like module (2017), Appl. Environ. Microbiol., 83, e01016-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.58 expressed in Escherichia coli Origami B(DE3) cells Corallococcus sp. EGB

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.58 E304A inactive Corallococcus sp. EGB
3.2.1.58 E309A inactive Corallococcus sp. EGB

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.58 laminarin + H2O Corallococcus sp. EGB most favorable substrate D-glucose + ?
-
 ?
3.2.1.58 pachyman + H2O Corallococcus sp. EGB
-
 D-glucose + ?
-
 ?
3.2.1.58 pustulan + H2O Corallococcus sp. EGB
-
 D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.58 Corallococcus sp. EGB
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.58 Ni-NTA column chromatography Corallococcus sp. EGB

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.58 laminarin + H2O most favorable substrate Corallococcus sp. EGB D-glucose + ?
-
 ?
3.2.1.58 pachyman + H2O
-
 Corallococcus sp. EGB D-glucose + ?
-
 ?
3.2.1.58 pustulan + H2O
-
 Corallococcus sp. EGB D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.58 ? x * 47040, calculated from amino acid sequence Corallococcus sp. EGB

Synonyms

EC Number Synonyms Comment Organism
3.2.1.58 beta-(1,3)(1,3)-glucanase
-
 Corallococcus sp. EGB
3.2.1.58 beta-(1,3)-glucanase
-
 Corallococcus sp. EGB
3.2.1.58 lamC
-
 Corallococcus sp. EGB
3.2.1.58 lamC the enzyme shows exo-beta-(1,3)/(1,6)-glucanase and displays and endo-beta-(1,4)-glucanase/xylanase activities Corallococcus sp. EGB
3.2.1.58 laminarinase
-
 Corallococcus sp. EGB

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.58 50
-
-
 Corallococcus sp. EGB

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.58 30 60 the enzyme shows about 70% activity at 30°C, about 75% activity at 35°C, about 85% activity at 40°C, about 90% activity at 45°C, 100% activity at 50°C, about 88% activity at 55°C, about 65% activity at 60°C, about 40% activity at 65°C, and about 25% activity at 70°C Corallococcus sp. EGB

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.58 50
-
 the enzyme retains more than 80% of its original activity after 30 min of incubation at 50°C Corallococcus sp. EGB

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.58 7
-
-
 Corallococcus sp. EGB

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.58 6 8 the enzyme shows more than 75% of activity between pH 6.0 and 8.0 Corallococcus sp. EGB

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.58 6 8 the enzyme retains more than 60% of its original activity after 30 min of incubation at pH 6.0-8.0 Corallococcus sp. EGB

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.58 Corallococcus sp. EGB isoelectric focusing
-
 5.3