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Literature summary extracted from

  • Huang, X.M.; Li, Q.Q.; Chen, X.L.; Fan, J.X.; Xu, X.H.; Sun, X.D.; Li, D.Y.; Zhao, H.X.
    Expression and characteristics of an endoglucanase from Trichoderma atroviride (TaEGII) in Saccharomyces cerevisiae (2017), Appl. Biochem. Biotechnol., 182, 1158-1170 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Saccharomyces cerevisiae Trichoderma atroviride

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Co2+
-
 Trichoderma atroviride
3.2.1.4 K+
-
 Trichoderma atroviride
3.2.1.4 Na+
-
 Trichoderma atroviride
3.2.1.4 Zn2+
-
 Trichoderma atroviride

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 raffinose Km value 0.011 mg/ml, pH 5.0, 60°C Trichoderma atroviride
3.2.1.4 additional information
-
 Avicel Km value 0.0122 mg/ml, pH 5.0, 60°C Trichoderma atroviride
3.2.1.4 additional information
-
 carboxymethylcellulose Km value 0.014 mg/ml, pH 5.0, 60°C Trichoderma atroviride
3.2.1.4 additional information
-
 cellobiose Km value 0.025 mg/ml, pH 5.0, 60°C Trichoderma atroviride

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Fe2+ 0.75 mM, 1.4fold stimulation Trichoderma atroviride
3.2.1.4 Mg2+
-
 Trichoderma atroviride
3.2.1.4 Mn2+
-
 Trichoderma atroviride

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Trichoderma atroviride A0A0C5H8Q7
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 proteolytic modification sequence contains a signal peptide of 21 amino acids Trichoderma atroviride

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 recombinant protein Trichoderma atroviride

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 119.9
-
 pH 5.0, 60°C Trichoderma atroviride

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 avicel + H2O
-
 Trichoderma atroviride ?
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Trichoderma atroviride ?
-
 ?
3.2.1.4 cellobiose + H2O
-
 Trichoderma atroviride 2 D-glucose
-
 ?
3.2.1.4 raffinose + H2O
-
 Trichoderma atroviride ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 44230, plus a signal peptide of 21 amino acids, calculated Trichoderma atroviride

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 EGII
-
 Trichoderma atroviride

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 60
-
-
 Trichoderma atroviride

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 60
-
 1 h, stable Trichoderma atroviride

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 0.091
-
 Avicel pH 5.0, 60°C Trichoderma atroviride
3.2.1.4 0.092
-
 raffinose pH 5.0, 60°C Trichoderma atroviride
3.2.1.4 0.094
-
 carboxymethylcellulose pH 5.0, 60°C Trichoderma atroviride
3.2.1.4 0.095
-
 cellobiose pH 5.0, 60°C Trichoderma atroviride

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5
-
-
 Trichoderma atroviride

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 3 6
-
 Trichoderma atroviride

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.4 Trichoderma atroviride calculated
-
 5

Expression

EC Number Organism Comment Expression
3.2.1.4 Trichoderma atroviride expression is repressed by glucose down
3.2.1.4 Trichoderma atroviride expression is induced by microcrystalline cellulose, bran, carboxymethyl cellulose, rice straw, and corn stalk up