Cloning and functional analysis of two flavanone-3-hydroxylase genes from Reaumuria trigyna
Zhang, H.; Zhao, L.; Wang, J.; Zheng, L.; Dang, Z.; Wang, Y.; Acta Physiol. Plant. 36, 1221-1229 (2014)
No PubMed abstract available
Data extracted from this reference:
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.11.9
gene F3H1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Escherichia coli, survival rate of the recombinant Escherichia coli strain expressing RtF3H2 is higher than that of the strain expressing RtF3H1 under salt and drought stresses; gene F3H2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Escherichia coli, survival rate of the recombinant Escherichia coli strain expressing RtF3H2 is higher than that of the strain expressing RtF3H1 under salt and drought stresses
Reaumuria trigyna
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Reaumuria trigyna
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.11.9
Reaumuria trigyna
W0S9B2
collected from the Eastern Alxa-Western Ordos area in Inner Mongolia, China
-
1.14.11.9
Reaumuria trigyna
W0SDE6
collected from the Eastern Alxa-Western Ordos area in Inner Mongolia, China
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.9
root
high transcript levels of gene RtF3H2 in roots and stems; high transcript levels of gene RtF3H2 in roots and stems
Reaumuria trigyna
-
1.14.11.9
seedling
;
Reaumuria trigyna
-
1.14.11.9
stem
high transcript levels of gene RtF3H1 in stems, high transcript levels of gene RtF3H2 in roots and stems; high transcript levels of gene RtF3H1 in stems, high transcript levels of gene RtF3H2 in roots and stems
Reaumuria trigyna
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
743971
Reaumuria trigyna
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.11.9
?
x * 39010, sequence calculation; x * 39520, sequence calculation
Reaumuria trigyna
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.11.9
F3H1
-
Reaumuria trigyna
1.14.11.9
F3H2
-
Reaumuria trigyna
1.14.11.9
flavanone-3-hydroxylase
-
Reaumuria trigyna
1.14.11.9
RtF3H1
-
Reaumuria trigyna
1.14.11.9
RtF3H2
-
Reaumuria trigyna
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.9
gene F3H1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Escherichia coli, survival rate of the recombinant Escherichia coli strain expressing RtF3H2 is higher than that of the strain expressing RtF3H1 under salt and drought stresses
Reaumuria trigyna
1.14.11.9
gene F3H2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Escherichia coli, survival rate of the recombinant Escherichia coli strain expressing RtF3H2 is higher than that of the strain expressing RtF3H1 under salt and drought stresses
Reaumuria trigyna
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Reaumuria trigyna
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.9
root
high transcript levels of gene RtF3H2 in roots and stems
Reaumuria trigyna
-
1.14.11.9
seedling
-
Reaumuria trigyna
-
1.14.11.9
stem
high transcript levels of gene RtF3H1 in stems, high transcript levels of gene RtF3H2 in roots and stems
Reaumuria trigyna
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
743971
Reaumuria trigyna
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.11.9
?
x * 39010, sequence calculation
Reaumuria trigyna
1.14.11.9
?
x * 39520, sequence calculation
Reaumuria trigyna
Expression
EC Number
Organism
Commentary
Expression
1.14.11.9
Reaumuria trigyna
transcript levels of the two F3H genes, RtF3H1 and RtF3H2, are increased in Reaumuria trigyna not only under salt stress but also under drought and cold stresses, and by abscisic acid; transcript levels of the two F3H genes, RtF3H1 and RtF3H2, are increased in Reaumuria trigyna not only under salt stress but also under drought and cold stresses, and by abscisic acid
up
General Information
EC Number
General Information
Commentary
Organism
1.14.11.9
evolution
the enzyme belongs to the 2-oxoglutarate-dependent dioxygenase (2-ODD) family; the enzyme belongs to the 2-oxoglutarate-dependent dioxygenase (2-ODD) family
Reaumuria trigyna
1.14.11.9
additional information
the 2S-naringenin substrate binding site of RtF3H1 consists of Ser135, Try149, Val214, Arg299, and Val304; the 2S-naringenin substrate binding site of RtF3H2 consists of Ser135, Phe149, Ile214, Arg299, and Ala305
Reaumuria trigyna
1.14.11.9
physiological function
the F3H gene, which encodes flavanone-3-hydroxylase, is an essential gene in the flavonoid biosynthetic pathway; the F3H gene, which encodes flavanone-3-hydroxylase, is an essential gene in the flavonoid biosynthetic pathway
Reaumuria trigyna
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.11.9
evolution
the enzyme belongs to the 2-oxoglutarate-dependent dioxygenase (2-ODD) family
Reaumuria trigyna
1.14.11.9
additional information
the 2S-naringenin substrate binding site of RtF3H1 consists of Ser135, Try149, Val214, Arg299, and Val304
Reaumuria trigyna
1.14.11.9
additional information
the 2S-naringenin substrate binding site of RtF3H2 consists of Ser135, Phe149, Ile214, Arg299, and Ala305
Reaumuria trigyna
1.14.11.9
physiological function
the F3H gene, which encodes flavanone-3-hydroxylase, is an essential gene in the flavonoid biosynthetic pathway
Reaumuria trigyna
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.11.9
Reaumuria trigyna
transcript levels of the two F3H genes, RtF3H1 and RtF3H2, are increased in Reaumuria trigyna not only under salt stress but also under drought and cold stresses, and by abscisic acid
up