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Literature summary extracted from

  • Zhang, H.; Zhao, L.; Wang, J.; Zheng, L.; Dang, Z.; Wang, Y.
    Cloning and functional analysis of two flavanone-3-hydroxylase genes from Reaumuria trigyna (2014), Acta Physiol. Plant., 36, 1221-1229 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.9 gene F3H1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Escherichia coli, survival rate of the recombinant Escherichia coli strain expressing RtF3H2 is higher than that of the strain expressing RtF3H1 under salt and drought stresses Reaumuria trigyna
1.14.11.9 gene F3H2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis, functional recombinant expression in Escherichia coli, survival rate of the recombinant Escherichia coli strain expressing RtF3H2 is higher than that of the strain expressing RtF3H1 under salt and drought stresses Reaumuria trigyna

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.9 a (2S)-flavan-4-one + 2-oxoglutarate + O2 Reaumuria trigyna
-
 a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.9 Reaumuria trigyna W0S9B2 collected from the Eastern Alxa-Western Ordos area in Inner Mongolia, China
-
1.14.11.9 Reaumuria trigyna W0SDE6 collected from the Eastern Alxa-Western Ordos area in Inner Mongolia, China
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.9 root high transcript levels of gene RtF3H2 in roots and stems Reaumuria trigyna
-
1.14.11.9 seedling
-
 Reaumuria trigyna
-
1.14.11.9 stem high transcript levels of gene RtF3H1 in stems, high transcript levels of gene RtF3H2 in roots and stems Reaumuria trigyna
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.9 a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
 Reaumuria trigyna a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.9 ? x * 39010, sequence calculation Reaumuria trigyna
1.14.11.9 ? x * 39520, sequence calculation Reaumuria trigyna

Synonyms

EC Number Synonyms Comment Organism
1.14.11.9 F3H1
-
 Reaumuria trigyna
1.14.11.9 F3H2
-
 Reaumuria trigyna
1.14.11.9 flavanone-3-hydroxylase
-
 Reaumuria trigyna
1.14.11.9 RtF3H1
-
 Reaumuria trigyna
1.14.11.9 RtF3H2
-
 Reaumuria trigyna

Expression

EC Number Organism Comment Expression
1.14.11.9 Reaumuria trigyna transcript levels of the two F3H genes, RtF3H1 and RtF3H2, are increased in Reaumuria trigyna not only under salt stress but also under drought and cold stresses, and by abscisic acid up

General Information

EC Number General Information Comment Organism
1.14.11.9 evolution the enzyme belongs to the 2-oxoglutarate-dependent dioxygenase (2-ODD) family Reaumuria trigyna
1.14.11.9 additional information the 2S-naringenin substrate binding site of RtF3H1 consists of Ser135, Try149, Val214, Arg299, and Val304 Reaumuria trigyna
1.14.11.9 additional information the 2S-naringenin substrate binding site of RtF3H2 consists of Ser135, Phe149, Ile214, Arg299, and Ala305 Reaumuria trigyna
1.14.11.9 physiological function the F3H gene, which encodes flavanone-3-hydroxylase, is an essential gene in the flavonoid biosynthetic pathway Reaumuria trigyna