Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Arbade, G.K.; Srivastava, S.K.
    Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of NAD synthetase from methicillin-resistant Staphylococcus aureus (2015), Acta Crystallogr. Sect. F, 71, 763-769 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
6.3.1.5 drug development enzymes involved in NAD metabolism and synthesis are significant drug targets as NAD is a central player in several cellular processes Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.5 gene nadE, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.1.5 purified recombinant His-tagged enzyme enzyme, hanging drop vapour diffusion method, mixing of 0.002-0.004 ml of 10 mg/ml protein in 20 mM Tris–HCl, pH 8.5, 200 mM NaCl, and 5% glycerol, with 0.002-0.004 ml of reservoir solution containing 200 mM CaCl2, 100 mM Tris–HCl, pH 7.5, 22% PEG 4000, and equilibration against 0.4 ml of reservoir solution, 20°C, 1 week, X-ray diffraction structure determination and analysis at 2.03 A resolution Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.5 Mg2+ required Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.5 ATP + deamido-NAD+ + NH3 Staphylococcus aureus
-
 AMP + diphosphate + NAD+
-
 ?
6.3.1.5 ATP + deamido-NAD+ + NH3 Staphylococcus aureus COL
-
 AMP + diphosphate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.5 Staphylococcus aureus Q5HEK9
-
-
6.3.1.5 Staphylococcus aureus COL Q5HEK9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.5 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.5 ATP + deamido-NAD+ + NH3
-
 Staphylococcus aureus AMP + diphosphate + NAD+
-
 ?
6.3.1.5 ATP + deamido-NAD+ + NH3
-
 Staphylococcus aureus COL AMP + diphosphate + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.5 NAD synthetase
-
 Staphylococcus aureus
6.3.1.5 NadE
-
 Staphylococcus aureus
6.3.1.5 NH3-dependent NAD+ synthetase
-
 Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.5 ATP
-
 Staphylococcus aureus

General Information

EC Number General Information Comment Organism
6.3.1.5 evolution the genes encoding the enzymes nicotinic acid mononucleotide adenylyltransferase (NaMNAT), NAD synthetase and NAD kinase responsible for the downstream conversion of nicotinic acid mononucleotide (NaMN) to NAD and NAD(P) are conserved in almost all investigated bacterial genomes Staphylococcus aureus
6.3.1.5 metabolism NAD synthetases are critical as they catalyze the amidation of nicotinic acid adenine dinucleotide (NaAD) to yield the enzyme cofactor NAD, which is the last step in NAD synthesis. NAD synthetase catalyzes the last step in the biosynthesis of nicotinamide adenine dinucleotide, making it a crucial intermediate enzyme linked to the biosynthesis of several amino acids, purine and pyrimidine nucleotides, coenzymes and antibiotics Staphylococcus aureus