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Literature summary extracted from

  • Koh, C.Y.; Siddaramaiah, L.K.; Ranade, R.M.; Nguyen, J.; Jian, T.; Zhang, Z.; Gillespie, J.R.; Buckner, F.S.; Verlinde, C.L.; Fan, E.; Hol, W.G.
    A binding hotspot in Trypanosoma cruzi histidyl-tRNA synthetase revealed by fragment-based crystallographic cocktail screens (2015), Acta Crystallogr. Sect. D, 71, 1684-1698 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.21 expressed in Escherichia coli BL21(DE3) cells Trypanosoma cruzi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.21 hanging drop vapor diffusion method, using 0.2 M lithium sulfate or ammonium sulfate, 26% (w/v) PEG 3350, 0.1 M bis-Tris pH 5.5, 1 mM TCEP Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.21 (6-bromopyridin-2-yl)methanol
-
 Trypanosoma cruzi
6.1.1.21 2-aminoquinolin-8-ol
-
 Trypanosoma cruzi
6.1.1.21 2-bromo-N-(quinolin-3-yl)acetamide
-
 Trypanosoma cruzi
6.1.1.21 N-(5-hydroxynaphthalen-2-yl)prop-2-enamide
-
 Trypanosoma cruzi
6.1.1.21 N-(quinolin-3-yl)acetamide
-
 Trypanosoma cruzi
6.1.1.21 N-(quinolin-3-yl)prop-2-enamide
-
 Trypanosoma cruzi
6.1.1.21 quinolin-3-amine
-
 Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.21 cytosol
-
 Trypanosoma cruzi 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis Trypanosoma cruzi
-
 AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.21 Trypanosoma cruzi Q4DA54
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.21 Ni-NTA column chromatography, Mono S cation-exchange column chromatography, and Superdex 200 gel filtration Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Trypanosoma cruzi AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.21 HisRS
-
 Trypanosoma cruzi
6.1.1.21 Histidyl-tRNA synthetase
-
 Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.21 ATP
-
 Trypanosoma cruzi

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.1.1.21 0.8
-
 at pH 7.5 and 25°C Trypanosoma cruzi 2-bromo-N-(quinolin-3-yl)acetamide
6.1.1.21 0.85
-
 at pH 7.5 and 25°C Trypanosoma cruzi N-(quinolin-3-yl)prop-2-enamide
6.1.1.21 1.65
-
 at pH 7.5 and 25°C Trypanosoma cruzi N-(5-hydroxynaphthalen-2-yl)prop-2-enamide
6.1.1.21 2
-
 IC50 above 2.0 mM, at pH 7.5 and 25°C Trypanosoma cruzi 2-aminoquinolin-8-ol
6.1.1.21 2
-
 IC50 above 2.0 mM, at pH 7.5 and 25°C Trypanosoma cruzi (6-bromopyridin-2-yl)methanol
6.1.1.21 2
-
 IC50 above 2.0 mM, at pH 7.5 and 25°C Trypanosoma cruzi N-(quinolin-3-yl)acetamide
6.1.1.21 2
-
 IC50 above 2.0 mM, at pH 7.5 and 25°C Trypanosoma cruzi quinolin-3-amine