EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.19.58 | gene mibH, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli, coexpression of mibH with the chaperones groES/EL and gene mibS | Microbispora sp. |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.19.58 | purified recombinant His6-tagged enzyme with bound FAD, hanging drop vapour diffusion method, mixing of 22 mg/ml protein in 20 mM HEPES, pH 8.0, and 150 mM KCl, with reservoir solution containing 12.5% MPD, 12.5% PEG 3350, 0.1 M MES/imidazole, pH 6.5, and 0.09 M NPS (1:1:1 NaNO3, Na2HPO4, (NH4)2SO4), X-ray diffraction structure determination and analysis at 1.85 A resolution | Microbispora sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.58 | L-tryptophan + FADH2 + Cl- + O2 + H+ | Microbispora sp. | - |
5-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
1.14.19.58 | L-tryptophan + FADH2 + Cl- + O2 + H+ | Microbispora sp. ATCC PTA-5024 | - |
5-chloro-L-tryptophan + FAD + 2 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.58 | Microbispora sp. | E2IHC5 | - |
- |
1.14.19.58 | Microbispora sp. ATCC PTA-5024 | E2IHC5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.19.58 | recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration | Microbispora sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.58 | L-tryptophan + FADH2 + Cl- + O2 + H+ | - |
Microbispora sp. | 5-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
1.14.19.58 | L-tryptophan + FADH2 + Cl- + O2 + H+ | - |
Microbispora sp. ATCC PTA-5024 | 5-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
1.14.19.58 | lantibiotic NAI-107-[tryptophan] + FADH2 + Cl- + O2 + H+ | NAI-107 acts as a peptidoglycan biosynthesis inhibitor by sequestering the cell wall precursor lipid II | Microbispora sp. | lantibiotic NAI-107-[5-chlorotryptophan] + FAD + 2 H2O | - |
? | |
1.14.19.58 | lantibiotic NAI-107-[tryptophan] + FADH2 + Cl- + O2 + H+ | NAI-107 acts as a peptidoglycan biosynthesis inhibitor by sequestering the cell wall precursor lipid II | Microbispora sp. ATCC PTA-5024 | lantibiotic NAI-107-[5-chlorotryptophan] + FAD + 2 H2O | - |
? | |
1.14.19.58 | additional information | structure-based analysis of the enzyme's substrate specificity, structure-function relationship, overview | Microbispora sp. | ? | - |
? | |
1.14.19.58 | additional information | structure-based analysis of the enzyme's substrate specificity, structure-function relationship, overview | Microbispora sp. ATCC PTA-5024 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.19.58 | mibH | - |
Microbispora sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.19.58 | 30 | - |
assay at | Microbispora sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.19.58 | 7.4 | - |
assay at | Microbispora sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.58 | FAD | - |
Microbispora sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.19.58 | evolution | structure comparisons reveal subtle amino acid differences within the MibH substrate binding site generates a solvent exposed crevice presumably involved in determining the substrate specificity of this unusual peptide halogenase | Microbispora sp. |
1.14.19.58 | physiological function | MibH is an FADH2-dependent Trp halogenase, that is only active when Trp is embedded within its cognate peptide substrate deschloro NAI-107 | Microbispora sp. |